纳米羟基磷灰石对成骨细胞功能代谢影响的研究

比较纳米羟基磷灰石(nHA)和常规羟基磷灰石(cHA)对成骨细胞功能代谢影响方面的差异。采用化学沉淀法制备nHA粉体,采用压制成型和无压烧结工艺制备nHA与cHA的块体材料。将Wistar乳鼠颅骨体外原代分离培养的成骨细胞接种于nHA与cHA的表面,分别在7、14、21、28d时检测细胞内总蛋白、ALP活性及细胞基质钙含量。结果表明,所制备的nHA与cHA的平均粒径分别为55nm和0.78μm;第21和28d,nHA表面附着的成骨细胞ALP活性和细胞基质钙含量均高于cHA。该研究提示:与相应的cHA比较,nHA更能增强成骨细胞的功能及代谢活动。

Experimental Research on the Effect of Nanophase Ceramics on Osteoblasts Functions

In order to study the cytocompatibility of nanophase hydroxyapatite ceramic in vitro, we prepared hydroxyapatite by use of the wet chemistry techniques. The grain size of hydroxyapatite of interest to the present study was determined by scanning electron microscopy and atomic force microscopy with image analysis software. Primary culture of osteoblast from rat calvaria was established. Protein content, synthesis of alkaline phosphatase and deposition of calcium-containing mineral by osteoblasts cultured on nanophase hydroxyapatite ceramics and on conventional hydroxyapatite ceramics for 7, 14, 21and 28 days were examined. The results showed that the average surface grain size of the nanophase and that of the conventional HA compact formulations was 55 (nanophase) and 780 (conventional) nm, respectively. More importantly, compared to the synthesis of alkaline phosphatase and deposition of calcium-containing mineral by osteoblasts cultured on nanophase was significantly greater than that on conventional ceramics after 21 and 28 days. The cytocompatibility was significantly greater on nanophase HA than on conventional formulations of the same ceramic.