荧光探针PCR检测肺炎支原体的临床应用研究

目的 探讨荧光探针PCR(FP-PCR)检测肺炎支原体DNA(MP-DNA)的临床应用价值.方法 563例疑似MP感染患儿为实验组,用FP-PCR检测MP-DNA,选取60例复查MP-DNA.同时应用颗粒凝集法检测肺炎支原体抗体(MP-Ab),1～2周后复查MP-Ab.同期选取20例无呼吸道感染者为对照组.结果 MP-DNA和MP-Ab的阳性率分别为34.99%、35.52%,两者差异无显著性(χ2=0.31,P>0.05),两种方法 一致率为97.69%.在疾病早期MP-DNA阳性率为30.48%,MP-Ab阳性率为10.16%,MP-DNA阳性检出率明显高于MP-Ab,差异有显著性(χ2=74.46,P<0.05).FP-PCR法敏感度及特异度分别是96.00%和98.62%.MP-DNA复查结果 与首次检查结果 及临床诊断一致.结论 FP-PCR法敏感性高,特异性强,方便快捷,稳定性好,适于临床应用,尤其适于MP早期诊断;且对MP既往感染有鉴别诊断意义.

Clinical application of fluorescent probe PCR for detection of mycoplasma pneumoniae

Objective To explore the clinical application of FP-PCR method to detect MP-DNA.Methods Five hundred and sixty-three children suspected of MP infection were enrolled in experimental group. FP-PCR was adopted to detect MP-DNA. MP-DNA was re-detected later in 60 children. At the same time,MP-Ab (MP antibody) was detected by means of particle agglutination. MP-Ab was re-detected one or two weeks later. Also 20 healthy children were selected as the control group. Results The positive rate of MP-DNA and MP-Ab were 34. 99% and 35.52% respectively,which showed no significant difference (x2 =0. 31, P > 0. 05). The coincidence of the two methods was 97. 69%. But the positive rate of MP-DNA was significantly higher than that of MP-Ab in the early stage(30. 48% vs 10. 16%) (x2 = 74. 46, P < 0. 05).The sensitivity and specificity of FP-PCR were 96. 00% and 98.62% respectively. The result of reviewed MP-DNA was consistent with the clinical diagnosis. Conclusion FP-PCR method is very sensitive, convenient and stable. It is fit for the clinical application ,especially the diagnosis of early MP infection. It helps to identify those who had been infected with MP before.