HPLC同时测定常山胡柚汁中柚皮芸香苷、柚皮苷和新橙皮苷的含量

目的建立同时测定常山胡柚(Citrus Changshan-huyou Y.B.Chang)果汁中柚皮芸香苷、柚皮苷和新橙皮苷含量的方法。方法采用HPLC法。色谱柱Diamnosil C18柱(200 mm×4.6 mm,5μm);检测波长283 nm;流动相为甲醇-水-冰醋酸(体积比为37∶63∶0.2);流速1.0 mL.min-1;柱温为室温。结果柚皮芸香苷、柚皮苷和新橙皮苷质量浓度分别在72～576 mg.L-1、113～904 mg.L-1和53～424 mg.L-1内与峰面积呈良好的线性关系,回归方程分别为A=6.790×103ρ+1.457×104,r=0.999 6(n=6);A=1.267×104ρ-2.366×104,r=0.999 9(n=6)和A=1.110×104ρ-1.472×105,r=0.999 9(n=6)。平均回收率分别为98.3%、98.0%和98.4%。测得样品中柚皮芸香苷、柚皮苷和新橙皮苷含量分别为157、289和172 mg.L-1。结论方法准确、可靠,可为常山胡柚的质量控制提供参考依据。

Simultaneous determination of contents of narirutin, naringin and neohesperidin in Citrus Changshan-huyou Y. B. Chang by HPLC

Objective To establish an HPLC method for simultaneous determination of narirutin, naringin and neohesperidin in Citrus Changshan-huyou Y. B. Chang. Methods The chromatographic method was carried out on a Diamnosil C₁₈ column ( 200 mm × 4.6 mm, 5 μm ) with a mobile phase of methanol-water-glacial acetic acid (V:V:V = 37:63:0.2). The flow rate was 1.0 mL·min^-1 and the column temperature was room temperature. The detection wavelength was set at 283 nm. Results The calibration curve was linear within the range of 72-576 mg·L^-1 (r = 0.999 6) for narirutin, 113-904 mg·L^-1 (r = 0.999 9) for naringin, and 53-424 mg·L^-1 (r = 0.999 9) for neohesperidin, respectively. The average recoveries of narirutin, naringin and neohesperidin were 98.3% (RSD = 1.53%, n = 6), 98.0% (RSD = 1.90%, n = 6), and 98.4% (RSD = 1.35%, n = 6), respectively. The average contents of narirutin, naringin and neohesperidin in Citrus Changshan-huyou Y. B. Chang were 157, 289 and 172 mg·L^-1, respectively. Conlusions The method is accurate and reproducible, and can be used for the quality control of Citrus Changshan-huyou Y. B. Chang.