Clonal diversity of Shiga toxin-producing Escherichia coli O157:H7/H- in Germany--a ten-year study |
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Authors: | Liesegang A Sachse U Prager R Claus H Steinrück H Aleksic S Rabsch W Voigt W Fruth A Karch H Bockemühl J Tschäpe H |
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Affiliation: | Robert Koch Institute, Wernigerode, Germany. |
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Abstract: | Two hundred and ten E. coli O157:H7/H- strains isolated from single cases and outbreaks of diarrhoea and haemolytic uraemic syndrome (HUS) in Germany between 1988 and 1998 were characterised by a range of molecular subtyping methods and phage typing in order to analyse their clonal nature. A high clonal heterogeneity, together with a considerable clonal stability, has been identified among the bacterial isolates and no single clonal type appeared to be geographically dominant. It is recommended to apply pulsed-field gel electrophoresis (PFGE) together with P gene profile determination (number and genomic positions of lambdoid bacteriophages) as laboratory tools for an extended epidemiological surveillance of E. coli OOFF phage typing will remain helpful as a first line of analysis, particularly in outbreak situations. |
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Keywords: | epidemiological surveillance pulsed-field gel electrophoresis P gene profile phage typing |
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