腰椎间盘突出新生血管的免疫细胞化学研究

应用免疫细胞化学的方法标记突出椎间盘新生血管的内皮细胞,从而研究浸润血管的发生、形态和分布.对84例从手术取得的椎间盘组织应用CD34标记血管内皮细胞,测定髓核血管内皮细胞生长因子(vascular endothelial growth factor,VEGF)免疫反应,按血管出现的多少分类为丰富、有、少或无.结果显示CD34标记中72例发现血管(出现率为85%),VEGF阳性反应78例.观察到血管网向髓核组织浸入,有时可见血管位于髓核细胞周围.表明在突出椎间盘髓核中观察到大量的新生血管,新生血管主要位于髓核纤维环和髓核细胞周围,提示新生血管可能影响髓核细胞的营养和代谢.VEGF参与血管的形成.

IMMUNOCYTOCHEMICAL STUDY ON NEONATAL BLOOD VESSELS IN LUMBAR INTERVERTEBRAL DISK HERNIA

The prevalence, morphology and topography of the vascular infiltration in the intervertebral disk hernia were studied through labeling the neonatal vascular endothelial cells by immunocytochemical method; vascular endothelial cells in the intervertebral disk tissues taken from 84 operations were labeled with CD34; the immune reactions of the vascular endothelial growth factor (VEGF) in nucleus pulposus blood vessels were detected and, according the number of vascular appearance, divided into four types: abundant, present, few and absent. The results showed that the CD34 labeling showed the blood vessels in 72 cases with 85 % and the positive VEGF reactions in 78 cases; the vascular net infiltration was found into the nucleus pulposus tissues; and the blood vessels could be seen surrounding the nucleus pulposus cells, suggesting that numbers of neonatal blood vessels were found in the herniated intervertebral disk nucleus pulposus and located about the fibrous rings and cells of nucleus pulposus; the neonatal cells could affect the nutrition and metabolism of nucleus pulposus cells; VEGF may participate in the vascularization.