不同产地菘蓝ISSR分析与鉴定

目的应用ISSR-PCR标记技术探讨不同产地菘蓝的遗传多样性,为其种质鉴定及育种提供参考。方法对采自全国不同地区的菘蓝及其变异类型的23份样本进行ISSR-PCR分析,采用NTSYS-pc软件计算Jaccard遗传相似系数,按非加权配对算术平均法(UPGMA)建立所研究类群的系统聚类图。结果 10条引物共扩增出109个条带,其中多态性条带为88条,占总扩增条带数的80.7%,菘蓝种质具有较丰富的遗传多样性。从聚类分析图可以看出,所研究样本聚为3支,第1支包括绝大多数样本,第2支为一被毛变异类型,第3支相距最远,从相似度0.62处即被分开,表明为一独立类群。结论 ISSR标记可以为菘蓝的种质资源鉴定、遗传关系分析及栽培育种提供分子生物学依据。

ISSR analysis and identification on Isatis indigotica from different habitats

Objective To study the genetic diversity and genetic relationship of Istais indigotica from different habitas by ISSR-PCR marker technique, so as to provide the reference for its germplasm identification and breeding. Methods Twenty-three samples of I. indigotica and its variation types from the different habitas of China were studied by ISSR-PCR markers. The Jaccard coefficient was worked out by NTSYS-pc software, and a cluster dendrogram of different samples was established based on the unweighted pair-group method with arithmetic mean (UPGMA). Results Ten ISSR primers generated 109 loci of which 88 loci were polymorphic. The average percentage of polymorphic bands (PPB) was 80.7%. The germplasm of I. indigotica had the plentiful genetic diversity. In the cluster dendrogram, the studied samples were clustered into three groups, the first one included majority samples, the second was the variation type with hair, and the third one was the farthermost, which was separated at the coefficient of 0.62. Conclusion ISSR method is suitable for the germplasm identification and genetic diversity analysis of I. indigotica, thus providing a biological theoretical foundation for its cultivation and breeding.