新斯的明对兔跨膈压和膈肌诱发电位的影响

本实验用家兔18只,动物麻醉后分离双侧膈神经,用30Hz的方波(0.2ms,10V)持续刺激膈神经致伤复制膈肌疲劳,分别测量伤前及伤后30min,60min.90min,120min的跨膈压及膈肌诱发电位(DEP)的变化.结果表明;刺激30min后最大跨膈压和诱发电位幅度明显降低,诱发电位潜伏期显著延长(n=7).膈肌疲劳后,静脉注射新斯的明0.05mg·kg~(-1),可迅速提高跨膈压和DEP幅度及缩短DEP潜伏期(n=6),而生理盐水组变化不明显(n=5).提示:(1)DEP可以作为评价膈肌疲劳的一项指标;(2)新斯的明有抗膈肌疲劳作用.

Effects of neostigmine on the transdiaphragmatic pressure and diaphragm evoked potential in rabbit

Experiments were performed on spontaneously breathing rabbits anesthetized with urethan. The diaphragmatic fatigue (DiF) was reproduced by the phrenic nerve stimulation (30 Hz, 10 V,0. 2 ms). The transdiaphragmatic pressure (Pdi) and diaphragm evoked potential (DEP) were measured before injury and after injury 30, 60, 90, and 120 min. Pdimax and the amplitude of DEP obviously decreased and the latency of DEP obviously delayed after injury 30min. The amplitude of Pdi and DEP were quickly promoted by introvenous injection of neostig-mine after DiF. The latency of DEP was partially recovered by introvenous injection of neostig-mine. It is concluded that neostigmine can improve the generation of the fatigued diaphragm. and promote its recovery.