萘甲异喹免疫抑制作用机制的研究

萘甲异喹是由粉防已碱改构的已被证实具有较强免疫抑制作用的化合物。本文进一步研究了该药的作用机制。结果表明：在淋巴细胞活化过程中，此药抑制细胞内钙调素含量增加和升高细胞内ｃＡＭＰ含量，并使淋巴细胞生成ＩＬ－２明显减少，从而抑制了淋巴细胞的增殖。

Immunosuppressive mechanism of naphthylmethyl isoquinoline

Naphthylmethyl isoquinoline(NI),a modified form of tetrandrine,was synthesized by China Pharmaceutical University.The previous experimental data showed that NI significantly inhibited the immunoreaction of mice and might be a immunosuppressive drug.In this study,We adopted MTT colorimetric analysiS,ELISA,radioimmunoassay and other methods and researched the mechanism of NI.The results showed that NI could restrain the increase of calmoduline(CaM) content and raise cAMP content in lymphocyte and decrease the output of IL-2 produced by lymphocyte,so that it inhibited the activation and proliferation of T and B lymphocytes,which indicates the immunosuppressive mechanism of NI is attributed to inhibiting the metabolism of CaM and cAMP in lymphocytes.