维生素E对创伤小鼠T细胞功能以及脂质过氧化和膜流动性的影响

观察了创伤小鼠Ｔ细胞丙二醛（ＭＤＡ）含量、膜流动性、Ｔ细胞功能的变化以及维生素Ｅ（Ｖ－Ｅ）的治疗作用。结果显示，创伤后Ｔ细胞ＭＤＡ含量增加；Ｔ细胞质膜，线粒体膜及微粒体膜流动性降低；Ｔ淋巴细胞转化、白介素２（ＩＬ－２）的产生、ＩＬ－２受体（ＩＬ－２Ｒ）的表达以及ＩＬ－２介导的淋巴细胞增殖反应均受抑。这些变化同ＭＤＡ的改变均密切相关。Ｖ－Ｅ（５０或１００ｍｇ·ｋｇ－１·ｄ－１，ｉｍ×４ｄ）可明显逆转各指标的变化。表明创伤后脂质过氧化反应是导致Ｔ细胞膜流动性降低及Ｔ细胞功能受抑的重要原因，而Ｖ－Ｅ则具有明显的治疗效果。

Effects of vitamin E on T cell lipid peroxidation, membrane fluidity and T cell functions in traumatized mice

Changes of T cell malondialdehgde (MDA)contents,membrane fluidity,T cell functions and therapeutic effects of vitamin E (V-E) were observed in traumatized mice.The results showed that T cell MDA contents were increased after trauma, membrane fluidity of T cell plasmalemma,mitochondria and microsome reduced and T lymphocytes transformation,interleukin 2(IL-2) production,IL-2 receptor(IL2R) expression and IL-2 mediated lymphocytes proliferation response were suppressed,which were related closely to MDA alteration.In vivo administration of V-E(50 or 100 mg/kg·d-1,im×4 d)could reverse above parameters, indicating lipid peroxidation after trauma is an important cause resulting in reduced T cell membrane fluidity and were suppressed T cell functions,on which V-E shows significant therapeutic effects.