内皮活化相关新基因EOLA1的亚细胞定位及组织表达

目的研究内皮细胞高表达脂多糖相关因子1(EOLA1)基因的亚细胞定位和组织表达特性，为对其进行进一步的功能研究打下基础。方法构建EOLA1-EGFP(绿色荧光蛋白)融合蛋白表达质粒，转染内皮细胞后瞬时表达，在激光共聚焦显微镜下观察EOLA1蛋白的亚细胞定位；通过人多组织Northern杂交研究EOLA1基因的组织表达。结果EOLA1蛋白在正常人各组织中呈选择性表达，在人的心脏、骨骼肌、肾脏、肝脏和胎盘组织中有较强的表达，在结肠、脾脏和小肠中有较弱的表达，而在脑、胸腺、肺和外周白细胞中无表达；EOLA1蛋白主要定位于细胞浆，少量表达于细胞核。结论EOLA1属胞内蛋白，可能在细胞内信号转导中起着作用。

Subcellular localization and tissue expression of a novel gene EOLA1

Objective To study the subcellular localization and the tissue expression of EOLA1(endothelial-overexpressed lipopolysaccharide-associated factor 1). Methods The fusion protein EOLA1-EGFP expressed vector was constructed and transfected into endothelial cells. After 24 hours posttransfection, the subcellular localization of EOLA1 was detected by laser-scanning microscopy. The tissue-specific distribution of EOLA1 was assessed with Multiple Tissue Northern Blots. Results The expression of EOLA1 was tissue-specific in various human tissues. With human multiple tissue Northern blot analysis, it was shown that EOLA1 could express in the heart, skeletal muscle, kidney, liver, placenta, colon, spleen, small intestine, but did not in the brain, lung, thymus, and peripheral blood leukocyte. EOLA1 was mainly localized in cytoplasm and could move into the nucleus. Conclusion EOLA1 is one of intercellular proteins and may play a role in intercellular signal transduction.