| 4-氨基吡啶对皮层神经元细胞内游离钙的影响 |
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| 引用本文： | 苏涛 陈盛强 邓维意 姜涛 丛文东 廖卫平. 4-氨基吡啶对皮层神经元细胞内游离钙的影响[J]. 现代临床医学生物工程学杂志, 2005, 11(2): 94-96 |
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| 作者姓名： | 苏涛 陈盛强 邓维意 姜涛 丛文东 廖卫平 |
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| 作者单位： | 广州医学院第二附属医院神经科学研究所 广州510260(苏涛，陈盛强，邓维意，姜涛，丛文东)，广州医学院第二附属医院神经科学研究所 广州510260(廖卫平) |
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| 摘 要： | 目的研究4-氨基吡啶(4-AP)对体外培养的皮层神经元细胞内游离钙浓度([Ca2+]i)的影响,了解4-AP的药理学作用机制.方法荧光探针Fluo-3-AM标记体外培养的皮层神经细胞内游离钙后,用共聚焦显微镜观察记录4-AP及L型谷氨酸对小鼠原代培养的皮层神经元[Ca2+]i的影响.结果4-AP与谷氨酸均能提高[Ca2+]i,两者峰值与持续时间存在差异,共同作用于细胞时的上升曲线与单用谷氨酸时相仿.结论4-AP的药理作用机制可能与提高神经细胞[Ca2+]i有关,其中机制与兴奋性氨基酸的[Ca2+]i升高作用可能不同.
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| 关 键 词： | 4-氨基吡啶 细胞内游离钙离子浓度 谷氨酸 共聚焦技术 皮层神经细胞 |
| 文章编号： | 1008-634X(2005)02-0094-03 |
| 修稿时间： | 2005-01-18 |
4-Aminopyridine-Induced Increases in Intracellular Free Ca~(2+) in Cultured Mouse Cortical Neurons |
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| SU Tao,CHEN Sheng-qiang,DENG Wei-yi,et al.. 4-Aminopyridine-Induced Increases in Intracellular Free Ca~(2+) in Cultured Mouse Cortical Neurons[J]. Journal of Modern Clinical Medical Bioengineering, 2005, 11(2): 94-96 |
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| Authors: | SU Tao CHEN Sheng-qiang DENG Wei-yi et al. |
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| Abstract: | ObjectiveTo analyze the effect of 4-aminopyridine (4-AP) on free intracellular calcium concentration (i) in cultured mouse cortical neurons and explore the possible pharmacological mechanism. MethodsApplying intracellular calcium probe Fluo-3-AM to indicate the changes of i; Using confocal microscopy to observe the calcium signal and the effect of 4-AP and L-glutamate on it. Results4-AP and glutamate could increase i significantly, whereas the calcium response curve to both reagents was characterized for each other. The i curve in response to simultaneous action of 4-AP and glutamate was similar to the effect of only glutamate. ConclusionThe pharmacological effects of 4-AP may be related with the complex change of calcium homeostasis, the underlying mechanism of which are possibly different from that of glutamate-induced change. |
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| Keywords: | 4-Aminopyridine i Glutamate Confocal Microscopy Cortical neurons culture |
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