| 红豆杉细胞提取物对癌细胞的抑制作用研究 |
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| 引用本文： | 鲁翠涛,梅兴国.红豆杉细胞提取物对癌细胞的抑制作用研究[J].中药材,2003,26(5):335-337. |
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| 作者姓名： | 鲁翠涛 梅兴国 |
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| 作者单位： | 1. 华中科技大学生命科学与技术学院,武汉,430074
2. 军事医学科学院毒物药物研究所,北京,100850 |
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| 基金项目： | 国家“九五”资助项目(96-C02-03-01)，国家863资助项目(102-12-06-01) |
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| 摘 要： | 目的：了解红豆杉细胞提取物对肝癌细胞系SMMC—7721生长的影响。方法：运用MTT比色法测定癌细胞的存活率和流式细胞仪分析细胞周期。结果：红豆杉细胞二氯甲烷提取物对癌细胞SMMC—7721的抑制作用远大于甲醇提取物，IC50值分别为0．0834mgDCWW．m1^-1和0．8002mgDCW．m1^-1；流式细胞仪分析发现外加红豆杉细胞二氯甲烷提取物后肝癌细胞G2／M期含量增加。结论：红豆杉细胞提取物在体外对肝癌细胞SMMC—7721的生长通过诱导凋亡而具有抑制作用。
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| 关 键 词： | 红豆杉 细胞提取物 癌细胞 抑制作用 研究 细胞周期 存活率 流式细胞仪 SMMC—7721 |
| 修稿时间： | 2002年11月29 |
Study on Anti-Tumor Activity of Extracts from Cultured Cells of Taxus chinensis |
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| Lu Cuitao,Mei Xingguo.Study on Anti-Tumor Activity of Extracts from Cultured Cells of Taxus chinensis[J].Jorunal of Chinese Medicinal Materials,2003,26(5):335-337. |
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| Authors: | Lu Cuitao Mei Xingguo |
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| Institution: | School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074. |
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| Abstract: | Objective:To analyze the cytotoxicity and inhibitory effect of extracts from cultured cells (from stems) of Taxus chinensis on cancer cell line SMMC-7721. Methods:MTT assay for cell viability and flow cytometry for cell cycle analysis. Results:MTT results showed that the extact by dichloromethane was more effective than that by methanol on SMMC-7721, IC50 were 0. 0834 mgDCW. ml-1 and 0. 8002 mg DCW. ml-1 respectively. SMMC-7721 cells in G2-M stage increased with higher concentration of dichloromethane extracts from Taxus chinensis cells and longer incubation. Conclusion:Extracts from cultured cells of Taxus chinensis could have cytotoxic effect on SMMC-7721 and could induce apoptosis of cancer cells. |
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| Keywords: | Taxus chinensis SMMC-7721 Cell toxicity Cell cycle |
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