| 紫草细胞培养生产紫草素的研究 |
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| 引用本文： | 胡立勇.紫草细胞培养生产紫草素的研究[J].中药材,2004,27(5):313-314. |
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| 作者姓名： | 胡立勇 |
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| 作者单位： | 广东药学院,广州,510220 |
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| 摘 要： | 目的:探索用紫草细胞生产紫草素的培养条件。方法:用正交试验考察紫草素生产培养基中L-苯丙氨酸、IAA、Ca(NO_3)_2·4H_2O的浓度对紫草素形成的影响;用摇瓶试验考察通气量对紫草素形成的影响。结果:紫草生产培养基中的L-苯丙氨酸、IAA、Ca(NO_3)_2·4H_2O的合适浓度分别为100、2、800 mg/L。通气量以150 r/min较为合适。结论:本研究可为紫草细胞培养生产紫草素提供数据。
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| 关 键 词： | 紫草 紫草素 细胞培养 |
| 修稿时间： | 2003年10月8日 |
Production of Shikonin by Cell Cultures of Lithospermum erythrorhizon |
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| Hu Liyong.Production of Shikonin by Cell Cultures of Lithospermum erythrorhizon[J].Jorunal of Chinese Medicinal Materials,2004,27(5):313-314. |
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| Authors: | Hu Liyong |
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| Institution: | Guangdong Pharmaceutical College, Guangzhou 510220. |
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| Abstract: | Objective:To explore cultural conditions of shikonin production by cell cultures of Lithospermum erythrorhizon. Method: Orthogonal design was applied in determination of shikonin within the medium. Flask test was applied in the study of shikonin production by the amount of ventilation. Result: The best medium consisted of 100mg/L L-phenylalanine, 2mg/L IAA and 800mg/L Ca(NO_3)24H_2O. The best amount of ventilation was get by shaken at 150r/min. Conclusion: This test provided data for producing shikonin by cell cultures of Lithospermum erythrorhizon. |
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| Keywords: | Lithospermum erythrorhizon Shikonin Cell cultures |
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