LC-MS／MS法测定心脑欣丸中甜菜碱的含量

目的建立心脑欣丸中甜菜碱的含量测定方法。方法采用LC—MS／MS法测定甜菜碱的含量，以电喷雾离子源（ESI）正离子模式将样品离子化，多反应监测（MRM）模式下对甜菜碱（m／z 118．20→57．92和118．20→59．00）进行测定。色谱柱为Venusil HILIC（100mm×2．1mm×3μm），流动相为乙腈-水（80：20），流速0.3mL·min^-1。结果甜菜碱浓度在4．44～177．6ng·mL^-1线性关系良好（r=0．9999），平均回收率为99．4％，RSD为1．5％（n=6）。结论该方法简便、可靠、准确，可作为心脑欣丸中甜菜碱的含量测定方法。

Determination of betaine in Xinnaoxin pills by LC-MS/MS

Objective To establish a quantitative method to determine the content of betaine in Xinnaoxin pills. Methods The content of betaine in Xinnaoxin pills was determined by LC-MS/MS. Eletrospray ionization （ESI） was used with positive source of the mass spectrometer and multiple reaction monitor （MRM） mode was used to detect betaine （m/z 118.20→57.92 and 118.20→59.00）. The chromatographic column was Venusil HILIC （100mm×2.1mm×3μm）. The mobile phase was acetonitrile - water （80： 20）, and the flow rate was 0.3mL·min^-1. Results The linear range ofbetaine was 4.44 -- 177.6 ng·mL^-1 （r = 0.999 9）. The average recovery was 99.4%, and RSD was 1.5% （n ： 6）. Conclusion This method is simple, accurate and can be used to determine the content of betaine in Xinnaoxin pills.