The interaction of influenza H5N1 viral hemagglutinin with sialic acid receptors leads to the activation of human γδT cells |
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作者姓名: | Yanlai Lu Zhen Li Chi Ma Hao Wang Jing Zheng Lianxian Cui Wei He |
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作者单位: | Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking UnionMedical College, State Key Laboratory of Medical Molecular Biology, Beijing, China |
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摘 要: | Highly pathogenic avian influenza H5N 1 epidemics are a significant public health hazard. Genetically engineered H5N 1 viruses with mammalian transmission activity highlight the potential risk of a human influenza H5N 1 pandemic. Understanding the underlying principles of the innate immune system in response to influenza H5N 1 viruses will lead to improved prevention and control of these potentially deadly viruses, γδT cells act as the first line of defense against microbial infection and help initiate adaptive immune responses during the early stages of viral infection. In this study, we investigated the molecular mechanisms of γδ T cells in response to influenza H5N1 viral infection, We found that recombinant hemagglutinin (rHA) derived from three different strains of influenza H5N 1 viruses elicited the activation of γδ T cells cultured in peripheral blood mononuclear cells (PBMCs). Both the cell surface expression of CD69, an early activation marker on γδ T cells, and the production of interferon-y (IFN-y) were significantly increased. Notably, the rHA protein-induced γδ T-cell activation was not mediated by TCRγδ, NKG2D or pattern recognition receptors (PRRs) or NKp46 receptors. The interaction of rHA proteins with sialic acid receptors may play a critical role in γδ T-cell activation. Our data may provide insight into the mechanisms underlyingγδT-cell activation in response to infection with H5N1 viruses.
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关 键 词: | 高致病性禽流感 H5N1病毒 T细胞活化 受体介导 相互作用 唾液酸 血凝素 外周血单核细胞 |
收稿时间: | 2013 Mar 28 |
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