Bcl-2及Bag-1基因产物在肾癌组织中的表达及意义

目的：讨论基因Bcl-2和Bag-1在肾癌组织中的表达及相互关系，了解肿瘤增殖与凋亡的特点。方法：采用免疫组化SABC法对41例肾癌与10例正常肾组织的石蜡标本切片对照，进行产物表达的统计研究，结果：Bcl-2在肾癌组织中阳性表达主为75．6％，与正常肾组织30．0％相比，有显著性差异（P＜0．05），但其表达与不同病理细胞类型，病理分级及临床分期无关（P＞0．05），Bag-1在肾癌组织中表达率为61．0％，正常肾组织10．0％，表达，Bag-1与肿瘤分级，分期及预后密切相关（P＜0．05），随肾癌恶性程度增加，其表这强度也增加，Bcl-2与Bag-1的表达正相关（r=0.438）。结论：肾癌组织中Bag-1表达可作为预后指标，Bcl-2可能参与肾组织由良性向恶性转化的过程，但与癌细胞恶性分化程度无关。

Expression and significance of Bcl-2 and Bag-1 in renal cell carcinoma

AIM To investigate Bcl 2 and Bag 1 expressions in renal cell carcinomas and to evaluate their clinical significance. METHODS Bcl 2 and Bag 1 expressions were studied with immunohistochemstry Streptavidin Biotin Peroxidase Complex (SABC) methods in 41 samples of renal carcinoma tissues and in 10 normal renal tissues, and their clinical significance were analysed. RESULTS The positive rate of Bcl 2 expression in the renal carcinoma tissues was 75.6%, while it was 30.0% in the normal renal tissues ( P <0.05). Ac cordingly, the positive rate of Bag 1 expression in the carcinoma tissues was 61.0%, it was 10.0% in the normal tissues ( P <0.05). However, Bcl 2 was not related to the tumor grade, clinical stage and pathological cell types. On the other hand, Bag 1 was positively related to the tumor grade, tumor stage and the prognosis of the patient ( P <0.05). Significant correlation was noted between Bcl 2 and Bag 1 ( r = 0.438). CONCLUSION Bag 1 might serve as a prognostic maker for the renal cell carcinoma. Bcl 2 might be involved in the transformation of benign to malignant process but is not related to the degree of differentiation of the tumor cells.