| PI3K/Akt信号通路对血管内皮功能的影响及促红素干预 |
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| 引用本文： | 邱清艳,秦俭,郑维平,高电萨,赵倩.PI3K/Akt信号通路对血管内皮功能的影响及促红素干预[J].第四军医大学学报,2009(22):2587-2590. |
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| 作者姓名： | 邱清艳 秦俭 郑维平 高电萨 赵倩 |
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| 作者单位： | 重庆医科大学附属第一医院心血管内科; |
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| 摘 要： | 目的：研究PI3K/Akt信号通路对心肌缺血后处理（I-PostC）大鼠血管内皮功能的影响及重组人促红细胞生成素注射液（rhEPO）的干预.方法：42只SD雄性大鼠随机分为7组：假手术组（Sham组）,缺血/再灌注（I/R）组（I/R组）,缺血后处理组（I-PostC组）,渥漫青霉素＋I-PostC组（Wort＋I-PostC组）,重组促红细胞生成素组（rhEPO组）,Wort＋rhEPO组,rhEPO＋I-PostC组.结扎大鼠冠状动脉左前降支建立I/R模型.RT-PCT检测各组主动脉组织中AktmRNA水平,取胸主动脉制成条片行离体器官灌流实验,测定其对去氧肾上腺素（PE）的收缩反应、对乙酰胆碱（Ach）的内皮依赖性舒张反应和对硝普钠（SNP）的非内皮依赖性舒张反应.结果：经I-PcostC和rhEPO的干预后,均能改善各组大鼠I/R损伤的血管内皮对乙酰胆碱的舒张反应,增加主动脉组织中AktmRNA的表达,但两者的上述效应差异无统计学意义;PI3K阻滞剂Wort可削弱两者的上述效应;联合应用I-PcostC,rhEPO与两者单独应用相比较差异不显著.结论：I-PostC,rhEPO干预,可通过激活PI3K-Akt信号通路发挥血管内皮保护作用.
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| 关 键 词： | 缺血/再灌注 缺血后处理 rhEPO 内皮功能 PI3K/Akt信号通路 |
Effects of PI3K/Akt signaling pathway on vascular endothelial function and interventional effects of erythropoietin |
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| QIU Qing-Yan,QIN Jian,ZHENG Wei-Ping,GAO Dian-Sa,ZHAO Qian.Effects of PI3K/Akt signaling pathway on vascular endothelial function and interventional effects of erythropoietin[J].Journal of the Fourth Military Medical University,2009(22):2587-2590. |
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| Authors: | QIU Qing-Yan QIN Jian ZHENG Wei-Ping GAO Dian-Sa ZHAO Qian |
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| Institution: | QIU Qing-Yan,QIN Jian,ZHENG Wei-Ping,GAO Dian-Sa,ZHAO Qian Department of Cardiology,First Affiliated Hospital,Chongqing Medical University,Chongqing 400016,China |
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| Abstract: | AIM:To investigate the effects of PI3K/Akt signaling pathway on vascular endothelial function in rats with myocardial ischemic postconditioning(I-PostC)and interventional effects of erythropoietin.METHODS:Forty-two male SD rats were randomized into 7 groups:Sham operation group(Sham group),ischemia/reperfusion group(I/R group),Ischemia-Postconditioning group(I-PostC group),Wortmannin+I-Post group(Wort+I-PostC group),rherythropoietin group(rhEPO group),Wort+rhEPO group and rhEPO+I-Postc group.Rat models with... |
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| Keywords: | ischemia/reperfusion ischemic-postconditioning rhEPO endothelial function PI3K/Akt signaling pathway |
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