| 小鼠胚胎成纤维细胞介导的IL-12抗S-180肿瘤作用 |
| |
| 引用本文： | 孙运芳.小鼠胚胎成纤维细胞介导的IL-12抗S-180肿瘤作用[J].第四军医大学学报,2009(24):2959-2962. |
| |
| 作者姓名： | 孙运芳 |
| |
| 作者单位： | 山东医学高等专科学校医学检验教研室; |
| |
| 基金项目： | 临沂市科技发展计划项目(080103055) |
| |
| 摘 要： | 目的：将IL-12重组载体转染入小鼠成纤维细胞,研究探讨转染后的抗肿瘤作用.方法：取小鼠胚胎获得原代成纤维细胞,应用PEI法将IL-12重组载体转染到小鼠胚胎成纤维细胞,同时设对照.建立肉瘤细胞（S-180）荷瘤小鼠模型,分别接种S-180细胞.于注射瘤细胞后定期给予转染后的成纤维细胞瘤体内注射,并设对照组.于致瘤后第21日处死小鼠,测肿瘤质量; ELISA法检测小鼠血清IL-12及γ-干扰素（IFN-γ）的表达; 检测NK（natural killer cells）细胞活性和脾淋巴细胞增殖情况、CD4/IFN-γ、CD8/IFN-γ双标记阳性细胞的克隆能力.结果：抑瘤率、NK细胞活性及淋巴细胞增生反应、血清IL-12、IFN-γ水平、CD4/IFN-γ及CD8/IFN-γ双标记阳性细胞的百分率均高于对照组（P〈0.01）.结论：转染后的IL-12重组质粒可进一步增强机体的免疫功能发挥其抗肿瘤的治疗作用.
|
| 关 键 词： | IL-12 成纤维细胞 S-180肉瘤 移植瘤 |
Anti-tumor effects of mouse embryonic fibroblasts-mediated interleukin-12(IL-12) on Sarcoma 180 xenografts |
| |
| SUN Yun-Fang.Anti-tumor effects of mouse embryonic fibroblasts-mediated interleukin-12(IL-12) on Sarcoma 180 xenografts[J].Journal of the Fourth Military Medical University,2009(24):2959-2962. |
| |
| Authors: | SUN Yun-Fang |
| |
| Institution: | SUN Yun-Fang Department of Clinical Laboratory,Shandong Medical College,Linyi 276002,China |
| |
| Abstract: | AIM:To investigate the anti-tumor effects of mouse embryonic fibroblasts-mediated interleukin-12(IL-12) on Sarcoma 180 xenografts.METHODS:The murine normal fibroblast were transfected with human IL-12 gene(FB/IL-12) by PEI.Controls were set up at the same time.The S-180 cells were inoculated in mice subcutaneously.Then FB/IL-12 was injected in mice in the same place subcutaneously at intervals,and controls were set up.On the 21st day,tumor weight was recorded.The serum levels of IL-12 and the expression of ... |
| |
| Keywords: | IL-12 fibroblasts Sarcoma-180 cancer cells tumor xenografts |
| 本文献已被 CNKI 维普 等数据库收录！ |
