| PI3K-Akt信号通路阻抑对结肠癌细胞LS-174T顺铂耐药逆转机制的研究 |
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| 引用本文： | 杨玉捷,孟辉,杨国嵘,薛永杰,郁蓉,贺雪娇,罗菲,惠玲,张方信.PI3K-Akt信号通路阻抑对结肠癌细胞LS-174T顺铂耐药逆转机制的研究[J].第四军医大学学报,2009(23):2739-2741. |
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| 作者姓名： | 杨玉捷 孟辉 杨国嵘 薛永杰 郁蓉 贺雪娇 罗菲 惠玲 张方信 |
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| 作者单位： | 兰州军区总医院消化科;兰州军区总医院胸外科;解放军第一医院病理科;兰州军区总医院医学实验中心; |
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| 基金项目： | 全军“十一五”课题(06MA089) |
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| 摘 要： | 目的：观察抑制磷脂酰肌醇3激酶和（或）蛋白激酶B（PI3K-Akt）信号通路对顺铂耐药结肠癌细胞株LS-174T细胞核中Y-Box结合蛋白1（Y-Box binding protein-1,YB-1）的活性的影响及肿瘤细胞顺铂耐药性的改变.方法：采用顺铂（cisplatin,DDP）药物浓度递增法、间歇性作用的体外诱导法建立人结肠癌的顺铂耐药细胞系LS-174T/DDP,药物Ly294002阻断PI3K-Akt信号通路传导后,采用Western Blot方法检测耐药肿瘤细胞磷酸化Akt表达水平,电泳迁移改变分析（EMSA）方法检测其核中转录因子YB-1活性的改变,MTT法检测DDP对细胞增殖的抑制率,流式细胞仪检测细胞凋亡.结果：阻断PI3K-Akt信号通路后,顺铂耐药细胞株中磷酸化Akt蛋白表达水平显著下降,同时其细胞核中YB-1的活性明显降低,且细胞的生长抑制率及细胞凋亡率均明显升高（P〈0.01）.结论：阻断PI3K-Akt信号通路能够提高顺铂耐药细胞株对顺铂的敏感性,PI3K-Akt信号通路可能通过激活YB-1在肿瘤顺铂耐药机制中发挥重要作用.
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| 关 键 词： | PI3K-Akt信号通路 Y-Box结合蛋白1 人结肠癌 获得性顺铂耐药 |
Effects of blocking PI_3K-Akt signal pathway in reversion of cisplatin resistance in colon adenocarcinoma cell line |
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| YANG Yu-Jie,MENG Hui,YANG Guo-Rong,XUE Yong-Jie,YU Rong,HE Xue-Jiao,LUO Fei,HUI Ling,ZHANG Fang-Xing.Effects of blocking PI_3K-Akt signal pathway in reversion of cisplatin resistance in colon adenocarcinoma cell line[J].Journal of the Fourth Military Medical University,2009(23):2739-2741. |
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| Authors: | YANG Yu-Jie MENG Hui YANG Guo-Rong XUE Yong-Jie YU Rong HE Xue-Jiao LUO Fei HUI Ling ZHANG Fang-Xing |
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| Institution: | YANG Yu-Jie1,MENG Hui2,YANG Guo-Rong3,XUE Yong-Jie3,YU Rong3,HE Xue-Jiao3,LUO Fei3,HUI Ling4,ZHANG Fang-Xing1 1Department of Gastroenterology,2Department of Cardiothoracic Surgery,4Center for Medical Experiment,Lanzhou General Hospital,Lanzhou Military Area Command,Lanzhou 730050,China,3Department of Pathology,No.1 hospital of PLA,Lanzhou 730030 |
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| Abstract: | AIM:To evaluate whether Ly294002,suppressing phosphatidylinositol 3 kinase PI3K/Akt survival signaling pathway,can affect the level of YB-1 activity in the nuclei of acquired cisplatin-resistant colon adenocarcinoma cell line and subsequently restore the sensitivity of acquired cisplatin-resistant colon adenocarcinoma cell line to cisplatin(DDP).METHODS:A LS-174T cell line resistant to cisplatin was successfully established by exposing cells to increasing cisplatin concentrations ranging from 0.1 to 0.8 mg/... |
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| Keywords: | PI3K/Akt pathway nuclear YB-1 human colon carcinoma acquired cisplatin resistance |
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