| Trastuzumab联合卡铂对过表达Her-2/neu食管癌细胞的抑制作用 |
| |
| 引用本文： | 邓勇军,张兰军,李海滨,杨鸿生,洪志鹏,李继梅.Trastuzumab联合卡铂对过表达Her-2/neu食管癌细胞的抑制作用[J].第四军医大学学报,2009(21):2285-2288. |
| |
| 作者姓名： | 邓勇军 张兰军 李海滨 杨鸿生 洪志鹏 李继梅 |
| |
| 作者单位： | 昆明医学院第一附属医院胸外科;中山大学肿瘤防治中心胸科;昆明医学院第一附属医院实验中心; |
| |
| 基金项目： | 云南省教育厅资助项目(08C0111) |
| |
| 摘 要： | 目的：探讨trastuzumab联合卡铂（CBP）对过表达Her-2/neu的食管癌细胞增殖的抑制作用.方法：常规培养食管癌细胞株EC109及TE3,采用流式细胞仪筛选出高表达Her-2/neu的食管癌细胞株EC109;将对数期生长的食管癌EC109细胞分为trastuzumab治疗组、CBP治疗组以及trastu-zumab联合CBP治疗组,分别采用不同工作浓度的trastuzum-ab、CBP以及trastuzumab联合CBP对相应实验组的EC109细胞给药,MTT法检测各组药物在不同工作浓度时对食管癌EC109细胞增殖抑制率,AnnexinV/PI双染色法检测肿瘤细胞凋亡率.结果：Trastuzumab单药、CBP单药或Trastuzumab联合CBP均能有效抑制EC109细胞的增殖,其抑制效应呈剂量依赖性（P〈0.05）,当药物工作浓度≥1PPC（血浆峰浓度,PPC）时,Trastuzumab联合CBP对EC109细胞的增殖抑制效应明显强于Trastuzumab单药组或CBP单药组,差异有统计学意义（P〈0.05）.Trastuzumab单药或CBP单药均能诱导EC109细胞凋亡,当药物工作浓度≥1PPC时，Trastuzumab联合CBP诱导的EC109细胞平均凋亡率明显高于Trastuzumab单药组或CBP单药组，差异有统计学意义（P〈0．05）．结论：Trastuzumab与卡铂联合应用具有协同效应，可以有效地抑制过表达Her-2／neu的食管癌EC109细胞增殖，诱导肿瘤细胞凋亡．
|
| 关 键 词： | 食管肿瘤 Her-2/neu Trastuzumab 卡铂 |
Antiproliferative effect of trastuzumab combined with carboplatin on Her-2-overexpressing esophageal carcinoma cells |
| |
| DENG Yong-Jun,ZHANG Lan-Jun,LI Hai-Bin,YANG Hong-Sheng,HONG Zhi-Peng,LI Ji-Mei.Antiproliferative effect of trastuzumab combined with carboplatin on Her-2-overexpressing esophageal carcinoma cells[J].Journal of the Fourth Military Medical University,2009(21):2285-2288. |
| |
| Authors: | DENG Yong-Jun ZHANG Lan-Jun LI Hai-Bin YANG Hong-Sheng HONG Zhi-Peng LI Ji-Mei |
| |
| Institution: | DENG Yong-Jun1,ZHANG Lan-Jun2,LI Hai-Bin1,YANG Hong-Sheng1,HONG Zhi-Peng1,LI Ji-Mei31Department of Thoracic Surgery,3Experiment Center,First Affiliated Hospital,Kunming Medical College,Kunming 650032,China,2Department of Thoracic Surgery,Cancer Center,Sun Yat-sen University,Guangzhou 510060 |
| |
| Abstract: | AIM:To investigate the antiproliferative effect of trastuzumab combined with carboplatin on her-2/neu-overexpressing esophageal carcinoma cells. METHODS:The esophageal carcinoma cell lines EC109 and TE3 were cultured routinely. The expression of her-2/neu of these cell lines was examined by flow cytometry and EC109 cells with her-2/neu-overexpressing were selected to be further studied. The EC109 cells in logarithmic phase were distributed into 3 groups. The first group was treated by trastuzumab alone,the ... |
| |
| Keywords: | esophageal neoplasm Her-2/neu trastuzumab carboplatin |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
