| 当归多糖对脐血造血细胞的抗冷冻损伤作用 |
| |
| 引用本文： | 关雪晶,杨慧,张雁,吴宏. 当归多糖对脐血造血细胞的抗冷冻损伤作用[J]. 医学争鸣, 2009, 0(22): 2549-2552 |
| |
| 作者姓名： | 关雪晶 杨慧 张雁 吴宏 |
| |
| 作者单位： | 重庆医科大学干细胞与组织工程研究室组织胚胎学教研室; |
| |
| 基金项目： | 重庆市自然科学基金(CSCT2007BB5284) |
| |
| 摘 要： | 目的：探讨当归多糖（APS）对脐血造血细胞的抗冷冻损伤作用.方法：采用细胞计数法比较羟乙基淀粉（HES）、明胶、淋巴细胞分离液（Ficoll）分离脐血单个核细胞（MNC）的回收率.应用细胞计数法、台盼蓝拒染法、集落形成实验和流式细胞术,检测冻存1,3,6mo脐血MNC的生物学活性,将脐血MNC与不同浓度APS共培养24h后冻存,1mo后复苏,观察APS对脐血MNC抗冷冻损伤的作用.结果：HES法、明胶法的MNC回收率均大于85%,显著高于Ficoll法（50.00±4.00）%（P〈0.05）;冻存1,3,6mo组MNC,CFU-Mix,CD34＋细胞回收率及台盼蓝拒染率差异均无显著性,且脐血MNC的损伤与冻存时间不相关.APS50,100,200,400mg/L不同浓度APS组MNC,CFU-Mix回收率和台盼蓝拒染率除APS400mg/L组下降外,其余各组明显上升（P〈0.05）;各组组间CD34＋细胞回收率差异无显著性.结论：APS可有效提高脐血造血细胞的抗冷冻损伤能力.
|
| 关 键 词： | 当归多糖 脐血 造血细胞 冷冻保存 |
Effect of Angelica polysaccharides on resistant ability of cryopreservation damage of UCB hematopoietic cells |
| |
| GUAN Xue-Jing,YANG Hui,ZHANG Yan,WU Hong Laboratory of Stem Cell , Tissue Engineering. Effect of Angelica polysaccharides on resistant ability of cryopreservation damage of UCB hematopoietic cells[J]. Negative, 2009, 0(22): 2549-2552 |
| |
| Authors: | GUAN Xue-Jing YANG Hui ZHANG Yan WU Hong Laboratory of Stem Cell Tissue Engineering |
| |
| Affiliation: | GUAN Xue-Jing,YANG Hui,ZHANG Yan,WU Hong Laboratory of Stem Cell and Tissue Engineering,Department of Histology and Embryology,Chongqing Medical University,Chongqing 400016,China |
| |
| Abstract: | AIM:To explore the effect on umbilical cord blood(UCB)hematopoietic cells of Angelica polysaccharides(APS)to resist cryopreservation damage.METHODS:Cytometry was used to compare the recovery rate of UCB mononuclear cells(MNC)when they were separated respectively by density gradient centrifugation of Ficoll,precipitation method of gelatinum and hydroxyethyl starch(HES);Cytometry,method of typan blue exclusion,colony-forming assay and flow cytometry were used to detect the bioactivity of UCB MNC after frozen ... |
| |
| Keywords: | Angelica polysaccharides(APS) umbilical cord blood hematopoietic cells cryopreservation |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
