| 携自杀基因微泡转染卵巢癌细胞株SKOV_3的超声参数及转染效率 |
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| 引用本文： | 樊萍,杨竹,王志刚,汪朝霞,甘胜伟,李攀. 携自杀基因微泡转染卵巢癌细胞株SKOV_3的超声参数及转染效率[J]. 医学争鸣, 2009, 0(22) |
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| 作者姓名： | 樊萍 杨竹 王志刚 汪朝霞 甘胜伟 李攀 |
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| 作者单位： | 重庆医科大学附属第二医院妇产科;重庆医科大学附属第二医院超声研究所;重庆医科大学解剖学教研室; |
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| 基金项目： | 国家自然科学基金面上项目(30973169); 重庆市教委科研基金(KJ070311) |
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| 摘 要： | 目的:探究超声介导携Ⅰ型单纯疱疹病毒胸苷激酶(hsv1tk)自杀基因微泡转染SKOV3细胞的超声参数及转染效率.方法:超声在不同微泡浓度与辐照时间(8,15,30,60s间隔1s)组合下辐照SKOV3细胞,MTT法筛选最适辐照时间和微泡浓度.将实验分成6组分别进行以下处理:超声辐照组,脂质体+超声辐照组,脂质体+微泡+超声辐照组,微泡+超声辐照组,裸质粒组(阴性对照),脂质体组(阳性对照).用裸质粒及脂质体处理后分别转染pcDNA3.1-EGFP/hsv1tk质粒;采用荧光显微镜、流式细胞技术分别定性和定量检测各组转染效率;采用逆转录聚合酶链反应(RT-PCR)方法检测hsv1tk基因的表达.结果:MTT检测在超声强度0.5W/cm2,频率1MHz,微泡浓度0.56×1011个/L,辐照时间8s间隔1s条件下,超声辐照微泡转染对细胞活性无明显抑制.经此条件转染后荧光显微镜下可观察到脂质体+微泡+超声辐照组的绿色荧光强度最强;流式细胞技术检测表明,微泡+超声辐照组转染效率为(11.74±0.19)%,比超声辐照组(2.19±0.22)%高,而脂质体+微泡+超声辐照组(25.62±0.08)%均高于其他各组(P<...
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| 关 键 词： | 超声参数 微泡造影剂 卵巢癌 转染效率 自杀基因 |
Ultrasound parameters and transfection efficiency of microbubble carrying suicide gene for transfecting ovarian cancer cell lines SKOV_3 |
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| FAN Ping,YANG Zhu,WANG Zhi-Gang,WANG Zhao-Xiao,GAN Sheng-Wei,LI Pan. Ultrasound parameters and transfection efficiency of microbubble carrying suicide gene for transfecting ovarian cancer cell lines SKOV_3[J]. Negative, 2009, 0(22) |
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| Authors: | FAN Ping YANG Zhu WANG Zhi-Gang WANG Zhao-Xiao GAN Sheng-Wei LI Pan |
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| Affiliation: | FAN Ping1,YANG Zhu1,WANG Zhi-Gang2,WANG Zhao-Xiao2,GAN Sheng-Wei3,LI Pan2 1Department of Obstetrics and Gynecology,2Ultrasound Study Institute,Second Affiliated Hospital,3Department of Anatomy,Chongqing Medical University,Chongqing 400010,China |
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| Abstract: | AIM:To investigate the ultrasound parameters and transfection efficiency of microbubble carrying herpes simplex virus thymidine kinase suicide gene(hsv1tk)for transfecting SKOV3 cells mediated by the ultrasound.METHODS:Under the combination conditions of different ultrasound exposure times(8,15,30,and 60 s with the interval as 1 s)and microbubbles at different concentrations,SKOV3 cells were exposed to the ultrasound so as to screen out the optimal ultrasound exposure time and microbubble concentration by 3... |
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| Keywords: | ultrasound parameters ultrasound microbubble contrast agents ovarian cancer transfection efficiency suicide gene |
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