| 顺铂通过活性氧协同抗hDR5抗体诱导食管癌细胞凋亡 |
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| 引用本文： | 刘广超,都景芳,刘世贵,龙章富,陶科,高荣,马远方. 顺铂通过活性氧协同抗hDR5抗体诱导食管癌细胞凋亡[J]. 医学争鸣, 2009, 30(23): 2768-2772 |
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| 作者姓名： | 刘广超 都景芳 刘世贵 龙章富 陶科 高荣 马远方 |
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| 作者单位： | 河南大学细胞与分子免疫学重点实验室,河南开封475000;四川大学生命科学学院生物资源与生态环境教育部重点实验室,四川成都610064;河南大学细胞与分子免疫学重点实验室,河南开封,475000;四川大学生命科学学院生物资源与生态环境教育部重点实验室,四川成都,610064 |
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| 基金项目： | 河南省杰出人才创新基金(0321001800) |
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| 摘 要： | 目的：探讨顺铂与抗死亡受体5（DR5）激动性抗体mDRA-6联合应用对食管癌细胞系EC9706细胞凋亡的影响及作用机制.方法：顺铂、抗体mDRA-6单独或联合作用于食管癌细胞,用流式细胞术检测药物的细胞毒作用、食管癌细胞表面DR5表达、细胞凋亡、细胞内活性氧水平、以及线粒体膜电位的变化;在荧光显微镜下观察细胞凋亡的形态学变化及DR5表达.结果：顺铂和抗体mDRA-6对食管癌细胞均具有细胞毒作用,并具有剂量依赖性,而且顺铂明显提高食管癌细胞对抗体mDRA-6细胞毒的敏感性.顺铂、抗体mDRA-6单独或联合应用均导致食管癌细胞呈现典型细胞凋亡特征.流式细胞术分析结果显示,mDRA-6和顺铂均诱导食管癌细胞凋亡,而且顺铂明显提高细胞对mDRA-6诱导细胞凋亡的敏感性;顺铂增强食管癌细胞表面及胞内的DR5表达,提高细胞内活性氧水平,降低线粒体膜电位.结论：顺铂主要通过活性氧激活细胞内线粒体细胞凋亡信号传导途径以及增强DR5表达,与抗体mDRA-6联合协同诱导食管癌细胞凋亡.研究结果对进一步探讨抗DR5激动性抗体及TRAIL的抗肿瘤临床应用有一定的指导意义.
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| 关 键 词： | DR5 激动性抗体 TRAIL 细胞凋亡 顺铂 |
Cisplatin augments apoptosis of esophageal carcinoma cells induced by agonistic anti-hDR5 antibody by DR5 and mitochondria death pathway |
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| LIU Guang-Chao,DU Jing-Fang,LIU Shi-Gui,LONG Zhang-Fu,TAO Ke,GAO Rong,MA Yuan-Fang Key Laboratory of Cellular , Molecular Immunology,Henan University,Kaifeng ,China,Key Laboratory of Bio-resources , Eco-environment,Ministry of Education,College of Life Science,Sichuan University,Chengdu. Cisplatin augments apoptosis of esophageal carcinoma cells induced by agonistic anti-hDR5 antibody by DR5 and mitochondria death pathway[J]. Negative, 2009, 30(23): 2768-2772 |
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| Authors: | LIU Guang-Chao DU Jing-Fang LIU Shi-Gui LONG Zhang-Fu TAO Ke GAO Rong MA Yuan-Fang Key Laboratory of Cellular Molecular Immunology Henan University Kaifeng China Key Laboratory of Bio-resources Eco-environment Ministry of Education College of Life Science Sichuan University Chengdu |
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| Affiliation: | LIU Guang-Chao1,2,DU Jing-Fang1,LIU Shi-Gui2,LONG Zhang-Fu2,TAO Ke2,GAO Rong2,MA Yuan-Fang1 1Key Laboratory of Cellular and Molecular Immunology,Henan University,Kaifeng 475000,China,2Key Laboratory of Bio-resources and Eco-environment,Ministry of Education,College of Life Science,Sichuan University,Chengdu 610064 |
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| Abstract: | AIM:To probe into the effect of cisplatin in combination with agonistic anti-hDR5 antibody mDRA-6 on apoptosis of human esophageal carcinoma cell line EC9706 and its mechanisms.METHODS:The cytotoxic action of mAb mDRA-6 and cisplatin on EC9706 cells,DR5 expression on EC9706 cell surfaces,apoptosis of EC9706 cells.Mitochondrial membrane potential and reactive oxygen species(ROS) were assessed by flow cytometry.The morphological changes and DR5 expression of EC9706 cells were observed by fluorencence microsco... |
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| Keywords: | DR5 agonistic antibody TRAIL apoptosis cisplatin |
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