| 高脂饮食大鼠肝脏脂肪性变及NPC1L1基因表达的变化 |
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| 引用本文： | 蒙广星,袁强,王毅军,孟化,夏琳,白飞虎. 高脂饮食大鼠肝脏脂肪性变及NPC1L1基因表达的变化[J]. 医学争鸣, 2009, 30(23): 2794-2797 |
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| 作者姓名： | 蒙广星 袁强 王毅军 孟化 夏琳 白飞虎 |
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| 作者单位： | 天津市第三中心医院肝胆外科,天津,300170;首都医科大学附属北京友谊医院胃肠外科,北京,100069;第四军医大学消化病研究所,陕西西安,710033;宁夏医科大学附属医院消化内科,宁夏银川,750000 |
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| 摘 要： | 目的：探讨NPC1L1及其胆固醇代谢相关基因在高脂饮食诱导肝脏脂肪性变中的可能作用.方法：实验大鼠分为普通饮食组及高脂饮食组,饲喂8周后取肝脏组织,酶法测定肝脏组织中总胆固醇（TC）、磷脂、高密度脂蛋白（HDL）、甘油三酯（TG）含量,RT-PCR检测肝脏组织中NPC1L1,SREBP-1c,FAS及ABCA1基因的表达变化,并在光镜及电镜下观察肝脏病理变化.结果：高脂饮食大鼠肝脏组织TC（67.6±3.1mg/gvs31.8±2.6 mg/g,P〈0.01）,TG（29.1±2.3 mg/gvs12.5±1.6 mg/g,P〈0.01）及磷脂（23.8±2.6 mg/gvs19.8±2.2 mg/g,P〈0.05）均较正常饮食大鼠增加.高脂饮食喂养组大鼠肝脏组织中胆固醇代谢相关基因NPC1L1（3.91±0.26,P〈0.01）,SREBP21c（2.84±0.23,P〈0.01）、FASmRNA（6.45±0.25,P〈0.01）表达水平显著高于正常饮食组,而ABCA1mRNA（0.56±0.18,P〈0.05）表达较正常饮食组降低.结论：肝脏组织NPC1L1表达增加及脂肪代谢相关基因SREBP-1c,FAS,ABCA1的表达
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| 关 键 词： | Niemann-Pick C1-Like1 肝脏脂肪性变 实时定量PCR |
Role of Niemann-Pick C1-Like 1 in hepatic steatosis induced by high cholesterol diet |
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| MENG Guang-Xing,YUAN Qiang,WANG Yi-Jun,MENG Hua XIA Lin,BAI Fei-Hu. Role of Niemann-Pick C1-Like 1 in hepatic steatosis induced by high cholesterol diet[J]. Negative, 2009, 30(23): 2794-2797 |
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| Authors: | MENG Guang-Xing YUAN Qiang WANG Yi-Jun MENG Hua XIA Lin BAI Fei-Hu |
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| Affiliation: | MENG Guang-Xing1,YUAN Qiang1,WANG Yi-Jun1,MENG Hua2 XIA Lin3,BAI Fei-Hu4 1Department of Hepatobiliary Surgery,Tianjin Third Center Hospital,Tianjin 300170,China,2Department of Gastrointestinal Surgery,Beijing Friendship Hospital,Capital Medical University,Beijing 100069,3Institute of Digestive Disease,Xijing Hospital,Fourth Military Medical University,Xi'an 710033,4Department of Digestive diseases,Affiliated Hospital of Ningxia Medical University,Yinchuan 750000 |
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| Abstract: | AIM:To determine the role of Niemann-Pick C1-Like 1(NPC1L1) and other genes correlated with cholesterol metabolism in hepatic steatosis induced by high cholesterol diet.METHODS:After being fed with high cholesterol diet for 8 weeks,rats were anaesthetized,livers were weighed and hepatic TC,TG,HDL and phospholipid were extracted and measured by enzymatic methods.Hepatic sterol regulatory element binding protein-1c(SREBP-1c),fatty acid synthase(FAS),ATP binding cassette transporter A1(ABCA1) and Niemann-Pick ... |
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| Keywords: | Niemann-Pick C1-Like 1 hepatic steatosis Real-time PCR |
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