乌拉坦增强延长离体大鼠心脏的低温保存效果

目的：观察乌拉坦对延长离体大鼠心脏的低温保存作用.方法：成年雄性SD大鼠分为两组（n=6）：对照组：HTK保存液; 试验组：HTK保存液加乌拉坦（30mmol/L）.心脏分别置入两种保存液4℃下保存6和18h.采用Langendorff心脏灌注装置进行保存前及保存后再灌注30min时心脏功能测定,比较心脏功能恢复率、ATP含量、细胞凋亡和超微结构变化.结果：低温保存后实验组左室功能恢复率较对照组明显升高,细胞凋亡及超微结构损伤较对照组减轻; 保存18h后实验组ATP含量显著高于对照组.结论：乌拉坦加入HTK心脏保存液能够显著增强离体大鼠心脏的低温保存效果.

Myocardial protective effects of urethane on isolated rat heart in prolonged hypothermic preservation

AIM：To observe the protective effects of urethane plus HTK on the isolated rat hearts in prolonged hypothermic preservation.METHODS：Male Sprague-Dawley rats（250-280 g） were divided into 2 groups（n=6 per group） according to each preservation solution used：control group,HTK solution;experimental group,HTK solution plus urethane.The hearts were arrested and stored in each preservation solutions respectively for 6 and 18 h at 4℃.After preservation,the myocardial function,ATP content,cardiomyocyte apoptosis and ultrastructure were examined.RESULTS：Compared with control group,experimental group showed higher recovery of cardiac function,lower rate of cardiomyocyte apoptosis and better cardiomyocyte ultrastructure both for 6 and 18 h of preservation.The ATP content after 18 h of preservation in control group was significantly higher than that in experimental group.CONCLUSION：Urethane led to significantly better and longer preservation in isolated hearts subjected to prolonged hypothermic preservation.