胸腺嘧啶核苷诱导肝癌HepG2细胞同步化

目的探讨胸腺嘧啶核苷（TdR）诱导肝癌HepG2细胞同步化的方法。方法于对数生长期的HepG2细胞中加入含终浓度为2．5mmoL／L的TdR培养28h后，PBS洗除TdR，加入新鲜血清培养基，此时记为0时刻，分别继续培养0、2、3、4、5、6、7、8、9、12、18、24、28h，收集细胞，同时实验设立对照组，采用流式细胞术检测细胞周期。结果分别于去除TdR后培养4、8、24h获得78．1％的S期细胞、67．2％的G2／M期细胞、86．3％的G1期细胞。结论终浓度为2．5mmoL／L的TdR处理肝癌HepG2细胞28h，再以新鲜培养基培养不同时间，可以获得同步化效果较好的S、G2／M和G1期细胞。

Synchronization of HepG2 cells induced by TdR

Objective To study method of synchronization in HepG2 cells induced by thymidine (TdR). Methods After HepG2 cells in the logarithm period were treated by 2.5 mmol/L TdR for 28 hours, the cells were washed twice using PBS to remove TdR and cultured for 0, 2, 3, 4, 5, 6, 7, 8, 9, 12, 18, 24 h continually. Cells were collected and cell cycle was detected by flow cytometry. Control cells were compared in experiment. Results 78.1% S-phase cells, 67.2% G2/M-phase cells and 86.3% G1-phase cells were obtained respectively after cells were removed from TdR and cultured for 4, 8, 24 h continually. Conclusions Cells treated by 2.5 mmol/L TdR for 28 hours and then cultured for different time in fresh culture medium shows a good synchronization at G1-phase, S-phase, G2/M-phase.