肝郁脾虚因素促进DEN诱发大鼠实验性肝癌的研究

目的：观察肝郁脾虚因素刺激对二乙基亚硝胺（DEN）诱发大鼠实验性肝癌发生及免疫功能的影响。方法：建立大鼠实验性肝癌肝郁脾虚证动物模型。大鼠给予诱癌剂（DEN70mg/kg灌胃，每周1次，连续8周），同时给予肝郁脾虚（束缚加饮食失节法）刺激至第19周，断头处死，做病理切片，HER染色，镜下观察肝组织病变；取肝组织用流式细胞术检测DNA含量；测定脾脏T，B淋巴细胞转化率和腹腔巨程式细胞吞噬中性红能力。结果：肝郁脾虚因素可明显降低该模型大鼠的体重和免疫功能并可使肝细胞DNA含量发生异常变化。结论：肝郁脾虚因素可促进DEN诱发大鼠实验性肝癌的发生发展。

Research on the Impact of Liver-stagnation and Spleen-defidency on Experimental Hepatocarcinoma Induced by DEN in Rats

Objective:To investigate the effect of liver-stagnation and spleen-deficiency on the immue function of rats and the contents of DNA in liver cells in rats of experimental hepatocarcinoma induced by DEN. Methods:Rats were stimulated with the factor of liver-stagnation and spleen-deficiency for 18weeks and simultaneously induced by DEN 70mg/kg once a week for 8 weeks. The pathological changes of liver and contents of DNA in liver cells were detected;T-lymphocyte transformation rate, B-lymphocyte transformation rate of the spleen and phagocytic function of peritoneal macrophage were tested. Results: Body weights of the rats could be decreased, the contents of DNA in liver cells could also be changed and the immune functions could be impaired significantly by stimulation of liver-stagnation and spleen-deficiency. Conclusion:The factor of liver-stagnation and spleen-deficiency could promote the development of experimental hepatocarcinoma induced by DEN in rats.