人结直肠癌裸小鼠原位移植肝转移模型的建立及其生物学特性的研究

建立人结直肠癌裸鼠原位移植肝转移模型,为探讨人结直肠癌肝转移机制和抗转移治疗提供实验工具。方法 采用组织学完整的人结直肠癌手术标本植入裸鼠结直肠壁内,观察原位移植的成瘤,移植瘤的侵袭和转移及其形态学特征。结果从３４例人结直肠癌标本中筛选出一株人结肠癌裸鼠直转移模型ＨＣＶ－ＭＨＮ－Ｉ,已传至１８代,一株人直肠癌裸鼠矸转移模型 ＨＲＡ,ＨＭＮ－２,已传至２１代。

Liver metastasis models of human colo-rectal carcinoma established in nude mice by orthotopic transplantation and their biologic characteristics

Objectives To establish a liver metastasis models of human colorectal carcinomas in nude mice. Methods Orthotopic transplantation of histologically intact colo rectal cancer tissues from patients into colo rectal mucosa of nude mice. Tumorgenicity, invasion, metastasis and morphological characteristics of the transplanted tumors were studied by light microscopy, electron microscopy and immunuhistochemistry. Results Liver metastasis models of human colon carcinoma (HCA HMN 1) and human rectal carcinoma (HRA HMN 2) were established after screening from 34 colorectal carcinomas. They had been passaged in vivo for 18 and 21 gererations respectively. There were lymphatic, hematogenous, and implanting metastases. CEA secretion was maintained after transplantation. The primary and liver metastatic tumors were similar to the original human carcinoma in histopathological and ultrastructural features, DNA content and chromosomal karyotype. Conclusion The liver metastasis models provide useful tools for the study of mechanism of metastasis and its treatment of human colo rectal cancer.