大鼠实验性胃溃疡自愈期间胰岛PP细胞的免疫组织化学研究

目的 探讨胰多肽在大鼠胃溃疡自愈期间的可能作用及意义.方法 应用免疫组织化学PAP法、形态计量和图像分析法,研究大鼠胃溃疡自愈期间胰岛PP细胞的形态学、细胞的面数密度(NA)及平均灰度变化.结果 与正常组和盐水组比较,胃溃疡组大鼠胰岛PP细胞NA于溃疡第6d明显下降,平均灰度值升高(P＜0.05);溃疡第10～28d,PP细胞NA升高、平均灰度值下降(P＜0.05);尤以第10d最明显.胰岛PP细胞免疫组织化学的形态观察结果与同期图像分析结果基本一致.结论 胰岛PP细胞在实验性胃溃疡自愈期间,其细胞NA和平均灰度均发生改变.提示PP细胞参与了胃溃疡自愈的调节过程.

IMMUNOHISTOCHEMICAL STUDY OF ISLET PP CELLS DURING THE HEALING PROCESS OF EXPERIMENTAL GASTRIC ULCER IN RATS

Objective To explore the possible function and significance of pancreatic polypeptide during the healing process of experimental gastric ulcer in rat. Methods The immunohistochemical PAP method,morphometry and image analysis were applied to study the changes of the morphology,numerical density on area(N_A) and mean grey degree of islet PP cells during the healing process of experimental gastric ulcer in rats. Results Compared with normal control group(NCG) and saline control group(SCG),the N_A of PP cells markedly decreased,and the mean grey degree markedly increased(P<0.05) on the 6th day after the experimental gastric ulcer group(EUG).During the 10th and the 28th day after the process of experimental gastric ulcer,the N_A of PP-positive cell increased,and the mean grey degree decreased(P<0.05),especially on the 10th day.The results of morphological observation and image analysis in pancreas islet PP cells were basically the same during the healing process of experimental gastric ulcer. Conclusion During the healing process of experimental gastric ulcer,the N_A and mean grey degree of PP-positive cell both changed.It indicated that PP cells played a role in the modulating process of experimental gastric ulcer.