NR4A2基因及其选择性剪接异构体与胃癌发生及转移的关系

目的:检测胃癌中NR4A2(又称Nurr1)基因的表达,从NR4A2调控机制出发寻找相关选择性剪接异构体,探讨其与胃癌发生和转移的关系。方法:通过选择性剪接数据库(ASD)预测NR4A2基因可能存在的选择性剪接异构体,半定量PCR检测NR4A2及异构体的表达,基因测序技术鉴定新的剪接异构体；实时定量PCR检测41例样本中NR4A2及剪接异构体的表达水平；免疫组织化学方法检测28例样本中NR4A2蛋白表达。结果:NR4A2基因在胃癌原位、癌旁、转移组织中均有表达,确定了2种剪接异构体NR4A2-Ⅰ和NR4A2-Ⅱ。实时定量PCR检测41例样本中NR4A2及2种异构体在癌旁组织表达水平高于原位癌 (P= 0.017,P=0.007,P=0.004)；在肝转移灶中表达水平低于原位癌(P=0.001,P=0.018,P=0.016),差异有统计学意义。免疫组化检测发现癌旁组织中NR4A2表达阳性率高于原位癌及转移灶,但差异无统计学意义(P=0.672)。结论:胃癌原位、癌旁和转移组织中至少存在NR4A2基因的2种剪接模式；NR4A2及异构体的表达变化与胃癌发生和转移相关。

NR4A2 and its splicing variants contribute to tumorigenesis and metastasis of gastric cancer

Objective:To investigate the expression of NR4A2(Nurr1) and its splicing variants in gastric cancer,and to evaluate their potential association with the pathogenesis and metastasis of gastric carcinoma.Methods:Alternative Splicing Database(ASD) was used to predict the possible splicing variants of NR4A2;they were identified by gene sequencing technique and their expression was detected by semi-quantitative PCR.The mRNA expression of NR4A2 and its splicing variants in tumor and the corresponding adjacent-tum...