| 数字化色谱指纹谱技术在六味地黄滴丸药材鉴定中的应用 |
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| 引用本文： | 安叡,朱丽萍,王莹,尤丽莎,王新宏. 数字化色谱指纹谱技术在六味地黄滴丸药材鉴定中的应用[J]. 中成药, 2010, 32(1): 3-6 |
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| 作者姓名： | 安叡 朱丽萍 王莹 尤丽莎 王新宏 |
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| 作者单位： | 上海中医药大学;浦东新区周浦医院; |
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| 基金项目： | 国家“十一五”科技支撑计划(2006BAI08B03-07) |
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| 摘 要： | 目的:采用RP-HPLC方法建立六味地黄滴丸和药材的数字化色谱指纹谱以用于该制剂中药材鉴定。方法:以马钱苷和丹皮酚为定性定量因子,采用相对保留值α为判据,建立RP-HPLC数字化指纹谱。InertsilODS-3:色谱柱;流动相是以甲醇-0.2%甲酸水溶液作梯度洗脱,流速是1mL/min;检测波长在240nm。结果:在选定的色谱条件下,建立了药材和制剂的数字化色谱-指纹谱HPLC-DFPS,提供了稳定可控的制剂指纹谱图,以及通过与空白样品比较探求了制剂中用以鉴定各药材的特征峰群。结论:结果表明利用数字化色谱指纹谱技术鉴定六味地黄滴丸中的药材是切实可行的。
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| 关 键 词： | 数字化色谱指纹谱 六味地黄滴丸 高效液相色谱法 |
Chromatograph-digitized fingerprint spectrum of Liuwei Dihuang Dripping Pill |
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| AN Rui,ZHU Li-ping,WANG Ying,YOU Li-sha,WANG Xin-hong. Chromatograph-digitized fingerprint spectrum of Liuwei Dihuang Dripping Pill[J]. Chinese Traditional Patent Medicine, 2010, 32(1): 3-6 |
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| Authors: | AN Rui ZHU Li-ping WANG Ying YOU Li-sha WANG Xin-hong |
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| Affiliation: | AN Rui1,ZHU Li-ping2,WANG Ying1,YOU Li-sha1,WANG Xin-hong1(1.Department of Chemistry,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China,2.Zhoupu Hospital,Shanghai 201318,China) |
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| Abstract: | AIM:To establish some HPLC-digitized fingerprint spectrum(HPLC-DFPS)in Liuwei Dihuang Dripping Pill preparation and to apply it to identification of medicinal materials.METHODS:Based on loganin and paeonoside contents adopted as qualitative and quantitative factors,relative retention value as creteria,RP-HPLC was carried out on inertsil ODS:column,mobile phase consisted of methanol-water(0.2% formyl acid),detection wavelength was set at 240 nm.RESULTS:Using above optimum chromatograph conditions,HPLC-DFPS o... |
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| Keywords: | digitized chromatography-fingerprint spectrum(DFPS) Liuwei Dihuang Dripping Pill HPLC |
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