DHPG和BMI诱导的癫痫样放电模型的建立及比较

目的 在离体大鼠海马脑片上,通过激活Ⅰ型代谢型谷氨酸受体(mGluR)或阻断γ-氨基丁酸(GABA)受体,诱导癫痫样放电.方法 将离体大鼠海马脑片暴露于Ⅰ型mGluR特异性激动剂对羟基苯甘氨酸(DHPG)或GABAA受体阻断剂荷包牡丹碱(BMI),利用全细胞记录模式记录海马CA3区域单个锥体细胞的电活动.结果 暴露于DHPG或BMI中的海马脑片CA3区域的锥体细胞均产生癫痫样放电,且两者的放电频率比较差异无统计学意义(P>0.05).结论 在离体情况下,破坏神经兴奋性和抑制性系统的平衡可以诱导产生癫痫样放电活动.

Establishment and comparison of DHPG and BMI induced epileptic discharge models

Objective To develop models of epileptic discharge by activating group Ⅰ metabotropic glutamate receptors (mGluR) or by blocking gamma-aminobutyric acid (GABA) receptors on rat hippocampal slices. Methods Rat hippocampal slices were exposed to mGluR group Ⅰ specific agonist dihydroxyphenylglycine (DHPG) or to GABAA receptor antagonists bicuculline methiodide(BMI), and single pyramidal cell in the CA3 region of the slice was recorded by whole cell patch clamp technique. Results Exposure to DHPG or BMI resulted in the induction of spontaneously occurring epileptic discharge in the CA3 region of rat hippocampal slice, and there was no significant difference in the frequency of discharge between them(P>0.05). Conclusion Epileptic discharge can be generated in vitro in response to a loss of balance between excitatory and inhibitory influences.