| 葛根素治疗大鼠脊髓缺血再灌注损伤的实验研究 |
| |
| 引用本文： | 田峰,傅永慧,李彬,萧万安,张志伟.葛根素治疗大鼠脊髓缺血再灌注损伤的实验研究[J].中国医科大学学报,2011,40(11):994-996. |
| |
| 作者姓名： | 田峰 傅永慧 李彬 萧万安 张志伟 |
| |
| 作者单位： | 1. 中国医科大学附属盛京医院骨外科,沈阳,110004
2. 喀左县人民医院骨科,辽宁喀左,028100 |
| |
| 基金项目： | 辽宁省教育厅高校科研计划(2008814) |
| |
| 摘 要： | 目的探讨葛根素治疗脊髓缺血再灌注损伤的最佳时间及机制。方法制作雄性SD大鼠脊髓缺血再灌注损伤模型，分别于损伤后1、2、4、6 h 腹腔内注射葛根素（50 mg/kg）。再灌注开始后48 h测试大鼠的运动功能，并测定硫氧还蛋白（Trx）表达水平及细胞凋亡指数。结果损伤后1、2、4 及6 h 葛根素组治疗组大鼠的运动功能有改善。缺血再灌注损伤导致Trx 表达水平下降，而葛根素可提高Trx-1和Trx-2mRNA 表达水平，并大幅降低细胞凋亡指数。结论脊髓缺血再灌注发生后6 h内给予葛根素可减轻缺血再灌注造成的损伤。葛根素的神经保护作用与Trx 表达水平的提高及细胞凋亡的减少有关。
|
| 关 键 词： | 缺血再灌注 葛根素 硫氧还蛋白 脊髓 细胞凋亡 |
The Effect and Mechanism of Puerarin Early Treatment of Spinal Cord Ischemia-reperfusion Injury |
| |
| TIAN Feng
,
FU Yong-hui
,
LI Bin
,
XIAO Wan-an
,
ZHANG Zhi-wei.The Effect and Mechanism of Puerarin Early Treatment of Spinal Cord Ischemia-reperfusion Injury[J].Journal of China Medical University,2011,40(11):994-996. |
| |
| Authors: | TIAN Feng FU Yong-hui LI Bin XIAO Wan-an ZHANG Zhi-wei |
| |
| Institution: | TIAN Feng1,FU Yong-hui1,LI Bin1,XIAO Wan-an1,ZHANG Zhi-wei2(1.Department of Orthopedic Surgery,Shengjing Hospital,China Medical University,Shenyang 110004,China,2.Department of Orthopedic Surgery,Kazuo Hospital,Kazuo 028100,China) |
| |
| Abstract: | Objective To explore the optimal therapeutic timing and mechanism of puerarin treatment of spinal cord ischemia-reperfusion injury.Methods The spinal ischemia-reperfusion injury was conducted in male Sprague-Dawley rats,and 50 mg/kg of puerarin was injected intraperitoneally at 1,2,4 and 6 h after the injury.Motor function was measured 48 h after reperfusion started.Thioredoxin(Trx) expression and apoptosis indices were determined.Results Improvement of motor function at 1,2,and 4 h was demonstrated in the ... |
| |
| Keywords: | reperfusion puerarin thioredoxin spinal cord apoptosis |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国医科大学学报》浏览原始摘要信息 |
| 点击此处可从《中国医科大学学报》下载免费的PDF全文 |
|
