Labile testosterone conjugate in human urine: further evidence. |
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| Authors: | J M Kjeld C M Puah G F Joplin |
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| Affiliation: | The Endocrine Unit, Royal Postgraduate Medical School, Hammersmith Hospital, London W12 OHS U.K. |
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| Abstract: | The origin of unconjugated testosterone released in human urine on storage and by dilute sulphuric acid treatment has been studied further. Various extraction procedures of urine have been employed and a new methodology involving anion exchange followed by acid hydrolysis and radioimmunoassay of the released unconjugated testosterone. Results indicate that human urine contains a very labile conjugate of testosterone and 5 alpha-dihydrotestosterone with polar solubility properties and a negative charge. |
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| Keywords: | Correspondence to J.M.K. present address: Landspítalinn University Hospital Department of Biochemistry Reykjavik Iceland. |
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