Identification of new defective interfering RNA species associated with porcine reproductive and respiratory syndrome virus infection |
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Authors: | Xiao Chao-Ting Liu Zhong-Hua Yu Xing-Long Ge Meng Li Run-Cheng Xiao Bo-Ren Zhou Hong-Rui |
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Affiliation: | College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, PR China. |
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Abstract: | In the present study, seven new defective interfering (DI) RNA species of porcine reproductive and respiratory syndrome virus (PRRSV) were identified. RT-PCR, Northern blot and sequence analyses indicated that these DI RNA specie have deletions of 8513-9176 nucleotides located between Nsp1/Nsp2 and Nsp10. Compared with the previous DI RNAs of PRRSV reported, they have three distinct characteristics: much smaller deletion sizes; different nucleotide repeats (2-12nt) used in the junction sites and in-frame deletions. The results further suggested that the similarity-assisted RNA recombination may be the main cause of generation of DI RNAs in PRRSV and probably in other arteriviruses. |
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