Cytometric investigations in histochemically identified neurons — Changes of fluorescence intensities and nuclear diameters in dopamine neurons of the arcuate nucleus in the cycling rat |
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Authors: | V. Schulz Dr. H. -G. Hartwig |
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Affiliation: | (1) Department of Anatomy and Cytobiology, Justus Liebig University, D-6300 Giessen, Germany;(2) Zentrum für Anatomie und Cytobiologie, Aulweg 123, D-6300 Giessen, Germany |
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Abstract: | Summary The relative formaldehyde-induced fluorescence intensities and the diameter of cell nuclei of fluorescent perikarya of the arcuate nucleus were recorded in serial cross-sectioned hypothalamic arcuate nuclei of regularly cycling rats (5 estrous and 4 proestrous animals). Stress-induced changes of cytometric parameters were avoided by preadaptation of animals to handling procedures. Dopamine neurons in a 75 m thick periventricular layer of the arcuate nucleus exhibited significantly smaller nerve cell nuclei and significantly reduced relative fluorescence intensities in proestrous rats. Both of these cytometric parameters indicate a decrease in the activity of periventricular dopamine neurons of the arcuate nucleus. The reported findings might support the hypothesis that dopamine inhibits the release of LH-RH.In partial fulfillment of requirements for an academic degree (Dr. med.)Supported by a grant from the Deutsche Forschungsgemeinschaft (-Ha 726/5-) and the Stiftung Volkswagenwerk (I/35 582) to H.G.H. |
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Keywords: | Rat Estrous cycle Hypothalamic arcuate nucleus Dopamine perikarya Cytometry |
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