H-89和wortmannin对forskolin和IBMX促进受损视网膜节细胞再生作用的影响

目的：探讨foirskolin和IBMX促进受损视网膜节细胞（RGCs）再生的作用机制。 方法：采用荧光逆行示踪标记术和自体坐骨神经移植术，研究蛋白激酶A（PKA0和抑制剂H－89或／和PI3－K特异抑制剂wortmannin玻璃体内注射对forskolin和IBMX促进成年金黄地鼠受损RGCs再生的影响。结果：H－89和wortmannin均可部分抑制forskolin和IBMX对受损RGCs的促再生作用，wortmannin的抑制作用更大；两药联合应用，可完全抑制forskolin和IBMX对受损RGCs的促再生作用。结论：forskolin和IBMX可能是通过PKA－CREB和P13－Akt通路实现对受损RGCs的促再生作用。

EFFECTS OF H-89 AND wortmannin ON THE PROMOTING EFFECTS OF forskolin AND IBMX ON THE REGENERATION OF INJURED RETINAL GANGLION CELLS

Objective The present study was aimed at investigating the regeneration promoting mechanism of forskolin and IBMX on injured retinal ganglion cells(RGCs). Methods Fluorescent retrograde labeling and autologus sciatic nerve transplanting techniques were used to observe the effects of H 89 or/and wortmannin on the regeneration promoting effects of forskolin and IBMX on injured retinal ganglion cells by intravitreal injection. Results Both H 89 and wortmannin could partly block the promoting effects of forskolin and IBMX on regeneration of injured RGCs, the effects of wortmannin might be more powerful, if united, they could inhibit the effects of forskolin and IBMX completely.Conclusion The regeneration promoting effects of forskolin and IBMX on injured RGCs might be realized by PKA CREB and PI3 K Akt accesses.