汉族人血小板GPⅡb HPA-3基因多态性与冠心病的相关性研究

目的： 观察血小板膜糖蛋白（GP）Ⅱb人类血小板抗原-3（HPA-3）基因多态性，在北京河北地区汉族人中各基因型的分布频率，分析该多态性与冠心病易感性的相关性。方法: 本研究采用病例对照设计，筛选符合冠心病、健康人入选标准的212例冠心病患者及106例健康对照人群为研究对象，记录冠脉造影病变支数，采用TaqMan探针技术检测HPA-3基因多态性。结果: 大于45岁的人群中，至少含有1个HPA-3b等位基因者较HPA-3a/3a原生型纯合子，冠心病人(72.4%/27.6%)多于健康人(57.1%/42.9%)，不同基因型在冠心病组和健康对照组两组间的分布具有显著差异(P<0.05)；冠心病患者不同病变支数基因型构成无显著差异(P＞0.05)。以是否患冠心病为因变量的二分类Binary Logistic回归分析，校正年龄、性别、体重指数对冠心病的影响后，结果显示HPA-3多态位点基因型与冠心病发病密切相关，含有至少1个HPA-3b等位基因者较HPA-3a/HPA-3a纯合型发生冠心病的危险性是2.105倍。结论: GPⅡb的HPA-3多态位点是汉族人冠心病发病的独立危险因素，尤其在年龄＞45岁的人群中表现明显。

Association between platelet membrane glycoprotein IIb polymorphism and coronary heart disease in Han people

AIM: To investigate the genotype distributions of HPA-3 in Han people from Beijing and Hebei province, and to study the association of the platelet glycoprotein IIb polymorphism with coronary heart disease (CHD).METHODS: Two hundred and twelve patients with coronary heart disease and 106 healthy controls were enrolled in this case-control study. The number of occlusive coronary artery was performed in all subjects. The genotypes of HPA-3 were determined by TaqMan probe technology. RESULTS: In CHD patients (older than 45 years) carriers of HPA-3b were over- represented compared with healthy controls (P<0.05). The prevalence of HPA-3 genetic variants had no difference in CHD patients of different numbers of occlusive coronary artery. Results were statistically evaluated with Binary Logistic regression, which showed the HPA-3 polymorphism was associated with an increased risk for CHD(OR: 2.105).CONCLUSION: The HPA-3 polymorphism of GP IIb is the risk factor of CHD in Han people, especially in patients older than 45 years.