| 茶多酚对微卫星不稳定结直肠癌裸小鼠的抗肿瘤作用及其作用机制 |
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| 引用本文： | 戴功建,金黑鹰,丁义江,夏建国,刘秀芳,刘飞,谈瑄忠,耿建祥.茶多酚对微卫星不稳定结直肠癌裸小鼠的抗肿瘤作用及其作用机制[J].中西医结合学报,2008,6(12):1263-1266. |
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| 作者姓名： | 戴功建 金黑鹰 丁义江 夏建国 刘秀芳 刘飞 谈瑄忠 耿建祥 |
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| 作者单位： | 1. 南京医科大学第一附属医院胃肠外科,江苏,南京,210082
2. 南京中医药大学第三附属医院,全国肛肠科医疗中心,江苏,南京,210001 |
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| 基金项目： | 国家自然科学基金资助项目，南京市医学发展重点项目资助，江苏省"科教兴卫工程"医学重点人才资助项目 |
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| 摘 要： | 目的：探索茶多酚对微卫星不稳定结直肠癌裸小鼠的作用及其作用机制。方法：采用hMSH2缺失结肠癌细胞株Lovo细胞建立裸鼠的皮下瘤模型，4周后使用皮下瘤接种法建立结肠造口结肠癌原位移植瘤模型。40只模型裸鼠分为模型组和50、75、100mg／kg茶多酚组。腹腔注射茶多酚治疗5d后，计算肿瘤抑制率，并检测肿瘤微卫星不稳定情况和转化生长因子β1（transforming growthfactor—β1，TGF-β1）、TGF-β2和胰岛素样生长因子（insulin—like growth factor，IGF）片段的变化情况。结果：使用50、75和100mg／kg 3个剂量茶多酚治疗后，在1、2、3和4周末，治疗组和模型组肿瘤抑制率比较，差异有统计学意义（P〈0．01）；3个治疗组之间比较，差异无统计学意义。使用茶多酚治疗后肿瘤微卫星标志趋于稳定。结论：茶多酚对错配修复基因缺失的结直肠癌有明显抑制作用，其作用机制可能与抑制微卫星不稳定有关。
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| 关 键 词： | 茶多酚 DNA错配修复 基因缺失 结直肠癌 微卫星不稳定性 裸小鼠 |
Anticancer effects of tea polyphenols on colorectal cancer with microsatellite instability in nude mice |
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| Gong-jian DAI,Hei-ying JIN,Yi-jiang DING,Jian-guo XIA,Xiu-fang LIU,Fei LIU,Xuan-zhong TAN,Jian-xiang GENG.Anticancer effects of tea polyphenols on colorectal cancer with microsatellite instability in nude mice[J].Journal of Chinese Integrative Medicine,2008,6(12):1263-1266. |
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| Authors: | Gong-jian DAI Hei-ying JIN Yi-jiang DING Jian-guo XIA Xiu-fang LIU Fei LIU Xuan-zhong TAN Jian-xiang GENG |
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| Institution: | Gong-jian DAI~1 Hei-ying JIN~2 Yi-jiang DING~2 Jian-guo XIA~2 Xiu-fang LIU~2 Fei LIU~2 Xuan-zhong TAN~2 Jian-xiang GENG~2 1.Department of Gastroenterology Surgery,the First Affiliated Hospital,Nanjing Medical University,Nanjing,jiangsu Province 210082,China 2.National Medical Center of Anorectal Surgery,the Third Affiliated Hospital,Nanjing University of Traditional Chinese Medicine,Jiangsu Province 210001,China |
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| Abstract: | Objective:To study the anticancer effects of tea polyphenols on colorectal cancer with microsatellite instability(MSI)in nude mice and to explore its mechanism. Methods:A colostomy was performed on the caecum of nude mice.Tumor fragments collected from the subcutaneous tumor of hMSH2-absence colon carcinoma Lovo cell line were surgically implanted onto the submucosa of the caecum during colostomy to establish the model.Then,the nude mice were divided into untreated group and 50,75 and 100 mg/kg tea polyphen... |
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| Keywords: | tea polyphenols DNA mismatch repair gene deletion colorectal neoplasms microsatellite instability nude mice |
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