钙蛋白酶10基因多态性与原发性高血压遗传易感性相关性研究

目的：探讨钙蛋白酶10基因多态性与原发性高血压遗传易感性的关系。方法：采用病例对照研究设计，以聚合酶链式反应-等位基因特异性扩增法（PCR-ASA），检测Calpain 10基因SNP43G／A位点和SNP44T／C位点基因型，并进行与原发性高血压的关联分析。结果：在非糖尿病人群中。原发高血压患者和血压正常者的钙蛋白酶10基因SNP43GG基因型频率分别为70．59％和72．73％，差异无统计学意义。其SNP44TT基因型频率分别为80．88％和78．41％，差异亦无统计学意义；分析原发性高血压与血糖、真胰岛素、C肽及胰岛素抵抗指数HOMA-IR等的关系，高血压患者与正常人群各项临床指标之间的差异无统计学意义（P〉0．05）。结论：钙蛋白酶10基因与昆明高原地区人群原发性高血压的发病无关，同时亦未发现原发性高血压与胰岛素抵抗的相关性。

STUDY ON THE ROLE OF CALPAIN10 GENE POLYMORPHISM IN THE GENETIC SUSCEPTIBILITY IN HYPERTENSION

Objective:To explore the hypothesis that Calpain10 gene contributes to the genetic susceptibility in hypertension in plateau population.Methods:In case control study,PCR-ASA method was used to determine the distribution of allele and genotype frequencies of SNP43 polymorphism(G/A)and SNP44 polymorphism(T/C)of calpain10 gene in 68 hypertensive patients and 88 normal control subjects.Results:The frequency of "GG"genotype of the SNP43 in hypertensive patients is as same as that in the control subjects;and so is the frequency of "TT"genotype of the SNP44.The variation of clinical metabolic parameter in hypertensive patients is also as same as that in the control subjects.Conclusion:The study suggested that Calpain10 gene is not associated with hypertension in plateau population in Kunming;and the correlation between hypertension and insulin resistance is not found either.