大肠腺癌组织MDR1表达与肠系膜淋巴结转移关系的研究

 目的　探索大肠腺癌组织多药耐药基因(MDR1)表达与大肠腺癌分化程度及肠系膜淋巴结转移之间的关系。方法　以光敏生物素标记MDR1 cDNA探针，链亲和素胶体金为检测系统做原位杂交(ISH-SAG）检测大肠癌MDR1 mRNA表达，同时用LSAB法对P-糖蛋白进行免疫组织化学检测。结果　大肠腺癌MDR1 mRNA表达阳性的肠系膜淋巴结转移率与MDR1表达阴性的肠系膜淋巴结转移率两者有显著性差异(P<0.05)，大肠癌MDR1表达阳性率与分化程度无显著性差异(P>0.05)。结论　MDR1表达高低与大肠腺癌肠系膜淋巴结转移呈正相关，MDR1阳性表达与细胞分化程度比较无统计学意义。MDR1表达的检测对判定化疗疗效及预后有一定的意义。

A Study on the Exspression of the Large Intestine Adenocarcinoma TissueMDR1 & the Relationship Between the Former and the Metastasis of the Lymphogylondulae Mesentericae

Objective This article probed into the relationship among the expression of the MDR1 large intestine adenocarcinoma tissue,the differentiation of the large intestine adenocarcinoma,and the metastasis of the lymphoglandulae mesentericae.Methods The 29 large intestine adenocarcinoma samples were measured by in situ hybridization with labeling MDR1 cDNA probe by sterptasiding-gold and immunohistchemistry technique (LSAB) with P-gp directed monoclondal antibody(JSB-1）.Results The metastatic rate of lymphoglandulae mesentericae has significant difference in patients with the expression of MDR1 from without expression of MDR1 (P<0.05).There was no significant association between the expression of MDR1 and differentiation of adenocarcinoma.Conclusion The MDR1 expression (whether high or low) is positive correlative with the metastasis of lymphoglandulae mesentericae.The positive expression of MDR1 is of no statistical significance compared with the differentiation of adenocarcinoma.To the determination of chemotherapeutic effect and prognosis,the measurement of MDR1 expression is somewhat of significance.