基于指纹图谱、模式识别及多成分同时定量的野菊花质量评价研究

目的建立HPLC指纹图谱、模式识别、多成分同时定量的质量评价模式及其在野菊花药材质量评价中的可行性及有效性分析。方法建立21批次野菊花HPLC指纹图谱,通过TCMYS指纹图谱软件对不同产地野菊花指纹图谱进行相似度分析、主成分分析及聚类分析,对指纹图谱中12个共有色谱峰进行指认,采用外标法对12种成分进行定量。结果 21批次野菊花HPLC指纹图谱相似度较高,有分类趋势,标定了17个共有峰,其中12个共有峰被指认为5-咖啡酰奎宁酸、绿原酸、咖啡酸、1,3-二咖啡酰奎宁酸、木犀草苷、4,5-二咖啡酰奎宁酸、3,5-二咖啡酰奎宁酸、3,4-二咖啡酰奎宁酸、蒙花苷、木犀草素和芹菜素和金合欢素,外标法线性关系良好(r≥0. 999 6),加样回收率均在95. 84%~102. 46%之间,RSD <2. 72%。结论 HPLC指纹图谱、模式识别、多成分同时定量的方法准确、可靠,为野菊花全面质量评价提供重要参考依据。

Quality Evaluation of Chrysanthemum indicum Based on Fingerprint,Pattern Recognition and Simultaneous Determination of Multi-Component

OBJECTIVE To establish a method based on HPLC fingerprint,pattern recognition and simultaneous determination of multi-component for quality evaluation of Chrysanthemum indicum,and investigate its feasibility and accuracy. METHODS HPLC fingerprints of 21 batches of Chrysanthemum indicum were established,similarity analysis,principal component analysis and cluster analysis were performed on fingerprints of Chrysanthemum indicum by TCMYS fingerprint software,and 12 common chromatographic peaks in fingerprints were identified,and 12 components were quantified by external standard method. RESULTS The HPLC fingerprints of 21 batches of Chrysanthemum indicum were similar,and their classification trend was found. Seventeen common peaks were found and 12 of them were identified as neochlorogenic acid,chlorogenic acid,caffeic acid,1,3-dicaffeoylquinic acid,cynaroside,isochlorogenic acid C,isochlorogenic acid A,( 1S,3R,4R,5R)-3,4-bis[[( E)-3-( 3,4-dihydroxyphenyl) prop-2-enoyl]oxy]-1,5-dihydroxycyclohexane-1-carboxylic acid,linarin,luteolin,apigenin and acacetin. Good linear relationship( r≥0. 999 6) was shown for the external standard method,the average recoveries were between 95. 84% and 102. 46%,and the RSDs were all below 2. 72%. CONCLUSION Combination of HPLC fingerprint,pattern recognition and simultaneous determination of multi-component are accurate and reliable for quality evaluation of Chrysanthemum indicum,which can provide important information for the overall quality evaluation of Chrysanthemum indicum.