HPLC法同时测定桑叶中芦丁、异槲皮苷、紫云英苷的含量

目的建立晾晒、烘干及鲜桑叶中芦丁、异槲皮苷、紫云英苷的含量测定方法。方法采用HPLC法,Shim pack ODS色谱柱(250 mm×4.6 mm,5μm),柱温35℃,流动相为0.4%磷酸乙腈溶液(A)和0.4%磷酸水溶液(B),梯度洗脱,检测波长为360 nm,流速设为1.0 ml.min-1。结果三种黄酮类成分在35 min内达到良好分离,芦丁在0.076～1.920 mg.L-1,异槲皮苷在0.086～2.148 mg.L-1,紫云英苷在0.042～2.108 mg.L-1范围内线性关系良好(r>0.999 3,n=6),三种成分平均加样回收率分别为99.7%(RSD=2.5%)9、8.8%(RSD=2.4%)和99.5%(RSD=4.1%)。结论该方法操作简单,结果准确,具有较好的重复性和稳定性,为评价桑叶药材炮制方法提供了一个很好的依据,同时也可用于桑叶药材的质量控制。

Simultaneous determination of rutin,isoquercetin,and astragalin in Mori Folium by HPLC

Aim To establish an HPLC method for the simultaneous determination of rutin,isoquercetin and astragalin in Mori Folium by natural drying,toasting,and unprocessed.Methods A high-performance liquid chromatography equipped a Shim pack ODS（250 mm×4.6 mm,5 μm）column with UV detection used.The mobile phase consisited of 0.4% phosphoric acid in acetonitrile（A）and 0.4% phosphoric acid in water（B）with gradient elution.The column temperature was 35℃,and the flow rate was 1.0 ml·min-1.The detection wavelength was set at 360 nm.Results The good saperation of three flavonoids was achieved within 35 min.Linear calibration curves were obtained with 0.076~1.920 mg·L-1 for rutin,0.086~2.148 mg·L-1 for isoquercetin,and 0.042~2.108 mg·L-1 for astragalin,（r0.999 3）.The average spiked recoveries of rutin,isoquercetin and astragalin were 99.7%（RSD=2.5%）,98.8%（RSD=2.4%）,and 99.5%（RSD=4.1%）,respectively.Conclusion The proposed method is simple,accurate,and repeatable,and can be used for quality control as well as a powerful tool to evaluate the processing of Mori Folium.