薄层色谱扫描法测定清肾颗粒中蒽醌类衍生物的含量

目的建立同时测定清肾颗粒中蒽醌类衍生物的含量测定方法。方法采用薄层扫描法对清肾颗粒中芦荟大黄素、大黄酸、大黄素、大黄素甲醚、大黄酚进行含量测定。展开剂为正己烷-乙酸乙酯-甲酸(30∶10∶0.5),扫描波长λS=435 nm,参比波长λR=650nm。结果芦荟大黄素的线性范围是0.023 6～0.118 1μg(r=0.998 13),平均回收率101.32%,RSD=2.44%(n=6);大黄酸的线性范围是0.060 0～0.300 0μg(r=0.998 61),平均回收率为101.31%,RSD=1.45%(n=6);大黄素的线性范围是0.045 0～0.225 0μg(r=0.998 41),平均回收率为100.82%,RSD=2.36%(n=6);大黄素甲醚线性范围是0.046 7～0.233 3μg(r=0.99681),平均回收率为100.87%,RSD=2.80%(n=6);大黄酚线性范围是0.032 5～0.162 5μg(r=0.997 56),平均回收率为105.35%,RSD=2.49%(n=6)。结论该方法简便、快捷、准确,可用于清肾颗粒中蒽醌类衍生物的含量测定。

Determination of aloe-emodin,rhein,emodin,chrysophanol,and physcion in qingshen granules by TLCS

Aim To establish a TLCS method for determinining galoe-emodin,rhein,emodin,chrysophanol,and physcion in qingshen granules.Methods Aloe-emodin,rhein,emodin,physcion,chrysophanol,were determined by TLC-scanning with a mixture of n-hexane-ethylacetate-formic acid（30∶ 10∶ 0.5）as the developing system.The detection wavelength was 435 nm.Results The Aloe-emodin was linear in the range of 0.023 6~0.118 1μg（r=0.998 13）,and the sample recovery was 101.32%,with RSD of 2.44%（n=6）.Rhein was linear in the range of 0.060 0~0.300 0 μg,（r=0.998 61）,and the sample recovery was 101.31%,with RSD of 1.45%（n=6）.Emodin was linear in the range of 0.045 0~0.225 0 μg,（r=0.998 41）,and the sample recovery was 100.82%,with RSD of 2.36%（n=6）.Physcion,was linear in the range of 0.046 7~0.233 3 μg,（r=0.996 81）,and the sample recovery was 100.87%,with RSD of 2.80%（n=6）.Chrysophanol was linear in the range of 0.032 5 μg~0.162 5 μg,（r=0.997 56）,and the sample recovery was 105.35%,with RSD of 2.49%（n=6）.Conclusion This method is simple,reliable and well repeatable.It could be used for content determination of the qingshen granules.