当归芍药散对痛经大鼠的作用及对精氨酸加压素含量的影响

目的探讨当归芍药散治疗痛经的作用及对精氨酸加压素（arginine vasopressin,AVP）系统的干预作用。方法采用催产素所致大鼠痛经模型,观察当归芍药散对痛经大鼠扭体次数的影响;采用酶联免疫吸附试验测定痛经模型大鼠子宫前列腺素F2α（prostaglandin F2α,PGF2α）的含量及下丘脑、血浆和子宫中AVP含量。结果当归芍药散能显著降低大鼠子宫PGF2α的水平,降低大鼠血浆AVP含量,升高大鼠下丘脑中AVP含量。结论当归芍药散对痛经大鼠AVP系统的干预作用可能是当归芍药散治疗痛经的机制之一。

Effects of Danggui Shaoyao Powders in Treating Dysmenorrhea and Its Effects on Contents of Arginine Vasopressin in Rats

Objective To observe the effects of Danggui Shaoyao Powders(DSP),a traditional Chinese medicine composed of angelica,peony,chuanxiong,poria cocos,alisma rhizoma,and atractylodes macrocephala,in the treatment of dysmenorrhea,and to explore its effects on the levels of arginine vasopressin(AVP).Methods The effects of DSP on twisting counts were observed in rats with dysmenorrhea induced by intraperitoneal injection of oxytocin.The contents of prostaglandin F2α(PGF2α) in the uterus,and AVP in the plasma,the hypothalamus and the uterus tissues were assayed by enzyme linked immunosorbent assay.Results DSP significantly reduced the contents of PGF2α in the uterus,and increased the contents of AVP in the hypothalamus,while reduced the contents of AVP in the rat uterine and plasma.Conclusion One of the mechanisms of DSP in treating dysmenorrhea is related with its intervening the AVP system in rats.