| 新藤黄酸体外抑制肺腺癌A549细胞增殖的作用 |
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| 引用本文： | 朱国旗,程卉,王训翠,王效山,李庆林. 新藤黄酸体外抑制肺腺癌A549细胞增殖的作用[J]. 安徽中医学院学报, 2011, 30(1): 53-56. DOI: 10.3969/j.issn.1000-2219.2011.01.020 |
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| 作者姓名： | 朱国旗 程卉 王训翠 王效山 李庆林 |
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| 作者单位： | 安徽中医学院科研实验中心,安徽,合肥,230038 |
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| 基金项目： | 安徽省高校自然科学研究项目，国家重大新药创制专项 |
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| 摘 要： | 目的研究新藤黄酸（Gambogenic acid,GNA）抑制肺腺癌细胞株A549增殖的作用及其相关机制。方法不同浓度GNA分别作用A549细胞24、48和72 h,甲基噻唑基四唑（methyl thiazolyl tetrazolium,MTT）比色法检测药物对A549细胞增殖的抑制率,吖啶橙/溴化乙啶（acridine orange/ethidium bromide,AO/EB）染色荧光显微镜检测细胞的凋亡;乳酸脱氢酶（lactate dehydrogenase,LDH）法检测GNA对A549细胞损伤作用。结果 MTT法检测结果显示,GNA在0-8μmol/L浓度范围内、24-72 h时间范围内对A549的生长有明显抑制作用,且呈浓度和时间依赖关系;相差显微镜观察表明GNA作用A549细胞48 h后,细胞变圆,呈半贴壁状态;AO/EB染色荧光显微镜观察显示GNA作用48 h后,细胞出现典型的凋亡特征;LDH实验进一步证实GNA在0-4μmol/L浓度范围内对正常细胞未见明显毒性作用。与空白对照组比较,8μmol/L GNA显著降低LDH释放。结论 GNA具有体外抑制肺腺癌细胞株A549增殖的作用,在0-4μmol/L浓度范围内GNA可能是通过诱导细胞凋亡而发挥抑制A549细胞增殖的作用。
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| 关 键 词： | 新藤黄酸 肺腺癌A549细胞 细胞凋亡 |
Inhibitive Effects of Gambogenic Acid on Proliferation of Lung Adenocarcinoma A549 Cells In Vitro |
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| ZHU Guo-qi,CHENG Hui,WANG Xun-cui,WANG Xiao-shan,LI Qing-lin. Inhibitive Effects of Gambogenic Acid on Proliferation of Lung Adenocarcinoma A549 Cells In Vitro[J]. Journal of Anhui Traditional Chinese Medical College, 2011, 30(1): 53-56. DOI: 10.3969/j.issn.1000-2219.2011.01.020 |
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| Authors: | ZHU Guo-qi CHENG Hui WANG Xun-cui WANG Xiao-shan LI Qing-lin |
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| Affiliation: | ZHU Guo-qi,CHENG Hui,WANG Xun-cui,WANG Xiao-shan,LI Qing-lin(Experimental Center for Scientific Research,Anhui College of Traditional Chinese Medicine,Anhui Hefei 230038,China) |
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| Abstract: | Objective To investigate anti-proliferation effect of Gambogenic acid(GNA) on lung adenocarcinoma A549 cells and its underlying mechanisms.Methods A549 cells were treated with different concentration of GNA for 24,48 and 72 h,respectively;the inhibitive effect was measured by methyl thiazolyl tetrazolium(MTT) assay;alternation of ultra structure was detected by AO/EB staining under fluorescent microscope;lactate dehydrogenase(LDH) assay was applied to determine whether GNA induced A549 cells necrosis.Result... |
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| Keywords: | Gambogenic acid lung adenocarcinoma A549 apoptosis |
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