| 孕酮诱导的蜕膜蛋白Depp在哺乳细胞株的转染表达与定位 |
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| 引用本文： | 陈应炉,朱恒锐,杨军,耿慧武,刘君,刘晓颖,范礼斌. 孕酮诱导的蜕膜蛋白Depp在哺乳细胞株的转染表达与定位[J]. 安徽医科大学学报, 2011, 46(2): 128-131 |
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| 作者姓名： | 陈应炉 朱恒锐 杨军 耿慧武 刘君 刘晓颖 范礼斌 |
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| 作者单位： | 安徽医科大学生物学教研室,合肥,230032;复旦大学遗传工程国家重点实验室,上海,200433 |
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| 基金项目： | 安徽省教育厅自然科学重点项目 |
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| 摘 要： | 目的 将孕酮诱导的蜕膜蛋白Depp基因构建到pCDGFP表达载体中,分析其在哺乳细胞株中的表达和定位情况.方法 用PCR方法扩增Depp全长序列,插入真核表达载体pCDGFP中,将构建好的质粒转染至COS7细胞,在荧光显微镜下观察其细胞定位;转染至HEK 293T细胞,提取总蛋白,利用GFP抗体进行Western bl...
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| 关 键 词： | 基因表达 转染 蜕膜 孕酮 蛋白质类 |
Human Depp was transfected for expression and localization in mammalian cell lines |
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| Chen Yinglu,Zhu Hengrui,Yang Jun,et al. Human Depp was transfected for expression and localization in mammalian cell lines[J]. Acta Universitis Medicinalis Anhui, 2011, 46(2): 128-131 |
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| Authors: | Chen Yinglu Zhu Hengrui Yang Jun et al |
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| Affiliation: | Chen Yinglu1,Zhu Hengrui2,Yang Jun1,et al(1Dept of Biology,Anhui Medical University,Hefei 230032,2State Key Laboratory of Genetic Engineering,Fudan University,Shanghai 200433) |
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| Abstract: | Objective To construct with pCDGFP expression vector for human wild Depp and investigate the expression and localization of the preliminary study in mammalian cell lines.Methods The full length fragment of Depp was used to amplify by PCR,then this fragment inserted into the eukaryotic expression vector pCDGFP to construct expression vector.Transfected into COS7 cells to observe localization under fluorescence microscope;transfected into HEK 293T cells,extracted total protein for Western blot to test GFP ant... |
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| Keywords: | gene expression transfection decidua progesterone proteins |
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