| E2F-1在胃腺癌细胞内质网应激反应中对Mcl-1的调控作用 |
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| 引用本文： | 王丽,侯丽丽,吴亚欧,桂丽,张旭东,张林杰.E2F-1在胃腺癌细胞内质网应激反应中对Mcl-1的调控作用[J].安徽医科大学学报,2011,46(4):332-336. |
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| 作者姓名： | 王丽 侯丽丽 吴亚欧 桂丽 张旭东 张林杰 |
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| 作者单位： | 安徽医科大学免疫学教研室,合肥,230032 |
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| 基金项目： | 国家自然科学基金，安徽省自然科学基金 |
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| 摘 要： | 目的 探讨Mcl-1在胃腺癌细胞对内质网应激诱导细胞凋亡耐受中的作用,以及内质网应激状态下E2F-1调控Mcl-1的机制.方法 采用PI单染法测定细胞凋亡率;Western blot检测GRP78、Mcl-1、Bcl-2、E2F-1蛋白变化;实时荧光定量PCR检测Mcl-1 mRNA水平变化;转染pcDNA -E2F-...
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| 关 键 词： | 胃肿瘤 腺癌 E2F1转录因子 内质网 衣霉素 细胞凋亡 转染 |
Regulation of Mcl-1 by E2F-1 in endoplasmic reticulum stress in gastric adenocarcinoma cells |
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| Wang Li,Hou Lili,Wu Yaou,et al.Regulation of Mcl-1 by E2F-1 in endoplasmic reticulum stress in gastric adenocarcinoma cells[J].Acta Universitis Medicinalis Anhui,2011,46(4):332-336. |
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| Authors: | Wang Li Hou Lili Wu Yaou |
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| Institution: | Wang Li,Hou Lili,Wu Yaou,et al(Dept of Immunology,Anhui Medical University,Hefei 230032) |
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| Abstract: | Objective To explore the role of Mcl-1 in resistance of gastric adenocarcinoma cells to endoplasmic reticulum(ER) stress-induced apoptosis and the mechanism of regulation of Mcl-1 by E2F-1 in the cells under ER stress.Methods Apoptosis was measured by flow cytometry using propidium iodide staining.Western blot was used to detect expression levels of various proteins including GRP78,Mcl-1,Bcl-2 and E2F-1.The expression of Mcl-1 at mRNA level was monitored by quantitative RT-PCR.cDNA(pcDNA-E2F-1-Flag)transfec... |
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