| pmCherry-N1-WWOX真核表达载体的构建及其在胆管癌细胞QBC939中的表达 |
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| 引用本文： | 朱凯,黄强.pmCherry-N1-WWOX真核表达载体的构建及其在胆管癌细胞QBC939中的表达[J].安徽医科大学学报,2011,46(4):328-332. |
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| 作者姓名： | 朱凯 黄强 |
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| 作者单位： | 安徽医科大学附属省立医院普外科,合肥,230001 |
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| 摘 要： | 目的 构建人脆性位点抑癌基因WWOX真核表达载体,并观察其在人胆管癌细胞株QBC939中的表达.方法 通过全基因合成的WWOX cDNA为模版,用PCR技术扩增,将扩增片段连接到PUC57质粒,构建克隆载体,鉴定出阳性克隆后用 DNA测序法鉴定重组质粒.将克隆载体与pmCherry-N1同时用Xho I/BamH I双...
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| 关 键 词： | 基因 肿瘤抑癌 基因表达 胆管肿瘤 |
Construction and identification of pmCherry-N1-WWOX eukaryotic vector and its expression in cholangiocarcinoma cell line QBC939 |
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| Zhu Kai,Huang Qiang.Construction and identification of pmCherry-N1-WWOX eukaryotic vector and its expression in cholangiocarcinoma cell line QBC939[J].Acta Universitis Medicinalis Anhui,2011,46(4):328-332. |
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| Authors: | Zhu Kai Huang Qiang |
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| Institution: | Zhu Kai,Huang Qiang(Dept of General Surgery,The Affiliated Provincial Hospital of Anhui Medical University,Hefei 230001) |
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| Abstract: | Objective To construct the eukaryotic expression vector of WW domain containing oxidoreductase(WWOX)gene and observe its expression in human cholangiocarcinoma cell line QBC939.Methods WWOX cDNA was whole-genome synthesized as a template for PCR.The fragment above was amplified with Xho I/BamH I double digestion and was cloned into the eukaryotic expression vector pmCherry-N1.The recombinant vector was identified by DNA sequencing.The expression of WWOX in recombinant plasmid transfected QBC939 cells was de... |
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| Keywords: | genes tumor suppressor gene expression bile duct neoplasms |
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