| sTRAIL真核表达载体的构建及其对裸鼠胶质瘤的治疗作用 |
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| 引用本文： | 李谷,马力,温良,龚江标,杨小锋,詹仁雅. sTRAIL真核表达载体的构建及其对裸鼠胶质瘤的治疗作用[J]. 浙江医学, 2008, 30(4): 324-327 |
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| 作者姓名： | 李谷 马力 温良 龚江标 杨小锋 詹仁雅 |
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| 作者单位： | 1. 浙江大学医学院附属第一医院神经外科,杭州,310003
2. 浙江大学医学院附属第二医院神经外科 |
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| 基金项目： | 浙江大学校科研和教改项目 |
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| 摘 要： | 目的构建重组基因表达载体PcDNA-sTRAIL并观察其对裸鼠种植性胶质瘤生长的抑制作用。方法通过PCR扩增和定向克隆技术构建重组真核表达载体PcDNA-sTRAIL。以阳离子脂质体介导转染裸鼠种植性胶质瘤细胞，同时设立未转染、空质粒转染及Lipofectin转染组，检测转染阳性率，并观察该基因载体对种植瘤生长的抑制作用。结果经过测序鉴定和蛋白表达检测证实，重组真核表达载体PcDNA-sTRAIL构建成功。阳离子脂质体介导质粒转染种植瘤瘤细胞阳性率达50％。脂质体PcDNA-sTRAIL转染种植瘤细胞后，相比未转染、空质粒转染及Lipofectin转染组，种植瘤体积、重量增长明显受抑制（均P〈0.05），表现出一定的肿瘤细胞增殖抑制作用。结论本研究成功构建了sTRAIL基因的真核表达载体，并从体外实验中初步证实了其对裸鼠种植性胶质瘤细胞增殖具有明显的抑制作用。
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| 关 键 词： | 胶质瘤 基因治疗 真核表达载体 脂质体 sTRAIL 真核表达载体 表达载体的构建 鼠胶质瘤 治疗 增殖抑制作用 mice glioma xenograft effect expression vector recombinant 细胞增殖 体外实验 基因载体 研究 肿瘤细胞 表现 增长 体积 脂质体介导 |
| 修稿时间： | 2008-01-28 |
Construction of eukaryotic recombinant sTRAIL expression vector and its effect on C6 glioma xenograft in athymic mice |
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| LI Gu,MA Li,WEN Liang,et al. Construction of eukaryotic recombinant sTRAIL expression vector and its effect on C6 glioma xenograft in athymic mice[J]. Zhejiang Medical Journal, 2008, 30(4): 324-327 |
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| Authors: | LI Gu MA Li WEN Liang et al |
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| Affiliation: | LI Gu,MA Li,WEN Liang,et al Department of Neurosurgery,The First Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310003,China |
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| Abstract: | Objective To construct eukaryotic recombinant sTRAIL expression vector and to investigate its effect on C6 glioma xenograft in athymic mice. Methods Eukaryotic recombinant sTRAIL expression vector PcDNA-sTRAIL was constructed by means of PCR and directed cloning. PcDNA-sTRAIL was transfected to C6 glioma cells mediated by cationic liposome and the positive transfection rate was detected by immunohistochemistry. C6 glioma xenografts in athymic mice were treated with PcDNA-sTRAIL plus lipofectin or blank vect... |
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| Keywords: | Glioma Gene therapy Eukaryotic recombinant expression vector Liposome |
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